Rabbit anti-Human, Mouse ESYT1 Polyclonal Antibody | anti-ESYT1 antibody

ESYT1 Antibody

Cat. Num. AAA9630359

• Gene Names: ESYT1; MBC2; FAM62A
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: ESYT1; Polyclonal Antibody; ESYT1 Antibody; E Syt1; E-Syt1; Esyt1; ESYT1_HUMAN; Extended synaptotagmin 1; Extended synaptotagmin like protein 1; Extended synaptotagmin-1; FAM62A; Family with sequence similarity 62 (C2 domain containing) member A; Family with sequence similarity 62 member A; KIAA0747; MBC2; Membrane bound C2 domain containing protein; Membrane-bound C2 domain-containing protein; Protein FAM62A; anti-ESYT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: ESYT1 Antibody detects endogenous levels of ESYT1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-ESYT1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human ESYT1.
• Conjugation: Unconjugated
• Similarity: Anchored to the endoplasmic reticulum membrane by a transmembrane hairpin structure; both N-terminus and C-terminus are cytoplasmic. The C2 domains mediate lipid and calcium binding. The N-terminal C2 domain binds calcium ions and is important for calcium-dependent lipid binding and interaction with membranes. Two calcium ions are bound at a high-affinity site and a third calcium ion is bound with lower affinity. May bind up to four calcium ions. In contrast, the second C2 domain apparently does not bind calcium (By similarity). The third C2 domain mediates interaction with membranes enriched in phosphatidylinositol 4, 5-bisphosphate and is required for translocation to the cell membrane in response to increased cytosolic calcium levels (PubMed:24183667 and PubMed:23791178). The SMP-LTD domain is a barrel-like domain that can bind various types of glycerophospholipids in its interior (By similarity). Belongs to the extended synaptotagmin family.
• Post Translational Modifications: Phosphorylated on Ser residues in insulin-treated adipocytes (in vitro); this promotes interaction with SLC2A4.
• Subunit Structure: Interacts (phosphorylated form) with SLC2A4 (By similarity). Interacts with ESYT2 and ESYT3.
• Tissue Specificity: Widely expressed.
• Function: Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (By similarity). Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane.
• Note: Localizes primarily to the endoplasmic reticulum. Recruited to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-ESYT1 antibody

Endoplasmic reticulum membrane; Multi-pass membrane protein; Cell membrane; Peripheral membrane protein; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 14149680
• NCBI GeneID: 23344
• NCBI Accession #: NP_056107.1
• NCBI GenBank Nucleotide #: NM_015292.2
• UniProt Accession #: Q9BSJ8; O94848; Q6PJN4; Q9H6J1; Q9H6W2; Q9Y416; A0FGR7; A8K2S2
• Molecular Weight: 124,003 Da
• NCBI Official Full Name: extended synaptotagmin-1 isoform 2
• NCBI Official Synonym Full Names: extended synaptotagmin-like protein 1
• NCBI Official Symbol: ESYT1
• NCBI Official Synonym Symbols: MBC2; FAM62A
• NCBI Protein Information: extended synaptotagmin-1; membrane-bound C2 domain-containing protein; family with sequence similarity 62 (C2 domain containing), member A
• UniProt Protein Name: Extended synaptotagmin-1
• Protein Family: Extended synaptotagmin
• UniProt Gene Name: ESYT1
• UniProt Synonym Gene Names: FAM62A; KIAA0747; MBC2; E-Syt1
• UniProt Entry Name: ESYT1_HUMAN

Uniprot Description

ESYT1: May play a role as calcium-regulated intrinsic membrane protein. Belongs to the extended synaptotagmin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: membrane; plasma membrane; integral to endoplasmic reticulum membrane

Molecular Function: protein binding; metal ion binding; phospholipid binding

Biological Process: lipid transport