Rabbit anti-Human, Mouse WAC Polyclonal Antibody | anti-WAC antibody

WAC Antibody

Cat. Num. AAA9630302

• Gene Names: WAC; Wwp4; BM-016; PRO1741
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: WAC; Polyclonal Antibody; WAC Antibody; 1110067P07Rik; BA48B24; BA48B24.1; BM 016; FLJ31290; KIAA1844; MGC10753; OTTHUMP00000019380; OTTHUMP00000019382; PRO1741; wac; WAC_HUMAN; WW domain containing adapter protein with coiled coil; WW domain containing adaptor with coiled coil; WW domain-containing adapter protein with coiled-coil; Wwp4; anti-WAC antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: WAC Antibody detects endogenous levels of WAC.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-WAC antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human WAC.
• Conjugation: Unconjugated
• Post Translational Modifications: Phosphorylated on tyrosine residues.
• Subunit Structure: Interacts (via coiled coil domain) with RNF20, RNF40 and UBE2A. Interacts (via WW domain) with RNA polymerase II. Interacts with MTOR and other components of the MTOR pathway including RPTOR, RUVBL1, RUVBL2, TTI1 and TTI2.
• Function: Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage. Positive regulator of amino acid starvation-induced autophagy. Also acts as a negative regulator of basal autophagy. Positively regulates MTOR activity by promoting, in an energy-dependent manner, the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex. This leads to the dimerization of the mTORC1 complex and its subsequent activation. May negatively regulate the ubiquitin proteasome pathway.
• Note: In distinct nuclear speckles. Colocalizes with pre-mRNA processing complexes.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-WAC antibody

Nucleus speckle; Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 18379328
• NCBI GeneID: 51322
• NCBI Accession #: NP_057712
• NCBI GenBank Nucleotide #: NM_016628.4
• UniProt Accession #: Q9BTA9; Q53EN9; Q5JU75; Q5JU77; Q5VXK0; Q5VXK2; Q8TCK1; A8K2A9; C9JBT9; D3DRW5; D3DRW6; D3DRW7
• Molecular Weight: Predicted: 71 kDa; ; Observed: 75 kDa
• NCBI Official Full Name: WW domain-containing adapter protein with coiled-coil isoform 1
• NCBI Official Synonym Full Names: WW domain containing adaptor with coiled-coil
• NCBI Official Symbol: WAC
• NCBI Official Synonym Symbols: Wwp4; BM-016; PRO1741
• NCBI Protein Information: WW domain-containing adapter protein with coiled-coil
• UniProt Protein Name: WW domain-containing adapter protein with coiled-coil
• Protein Family: Fibritin
• UniProt Gene Name: WAC
• UniProt Synonym Gene Names: KIAA1844
• UniProt Entry Name: WAC_HUMAN

NCBI Description

The protein encoded by this gene contains a WW domain, which is a protein module found in a wide range of signaling proteins. This domain mediates protein-protein interactions and binds proteins containing short linear peptide motifs that are proline-rich or contain at least one proline. This gene product shares 94% sequence identity with the WAC protein in mouse, however, its exact function is not known. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]

Uniprot Description

WAC: Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1). Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription. Regulates the cell-cycle checkpoint activation in response to DNA damage. Positive regulator of amino acid starvation-induced autophagy. May negatively regulate the ubiquitin proteasome pathway. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 10p12.1|10p12.1-p11.2

Cellular Component: nucleoplasm; spliceosome; nuclear speck; nucleus

Molecular Function: protein binding; chromatin binding

Biological Process: histone monoubiquitination; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; response to DNA damage stimulus; positive regulation of macroautophagy