Rabbit KDM3A Polyclonal Antibody | anti-KDM3A antibody

KDM3A Antibody

Cat. Num. AAA9630267

• Gene Names: KDM3A; TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: KDM3A; Polyclonal Antibody; KDM3A Antibody; DKFZp686A24246; DKFZp686P07111; JHDM2A; JHMD2A; JmjC domain-containing histone demethylation protein 2A; JMJD1; JMJD1A; Jumonji C domain containing histone demethylase 2A; Jumonji domain containing 1; Jumonji domain containing 1A; Jumonji domain containing protein 1A; Jumonji domain-containing protein 1A; Kdm3a; KDM3A_HUMAN; KIAA0742; Lysine (K) specific demethylase 3A; Lysine-specific demethylase 3A; Testis specific protein A; TSGA; anti-KDM3A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: KDM3A Antibody detects endogenous levels of KDM3A.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-KDM3A antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human KDM3A.
• Conjugation: Unconjugated
• Similarity: The JmjC domain and the C6-type zinc-finger are required for the demethylation activity. Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors. Belongs to the JHDM2 histone demethylase family.
• Function: Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TNP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
• Note: Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-KDM3A antibody

Cytoplasm; Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 308153659
• NCBI GeneID: 55818
• UniProt Accession #: Q9Y4C1; Q53S72; Q68D47; Q68UT9; Q6N050; Q8IY08; D6W5M3
• Molecular Weight: 1321
• NCBI Official Full Name: Lysine-specific demethylase 3A
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 3A
• NCBI Official Symbol: KDM3A
• NCBI Official Synonym Symbols: TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A
• NCBI Protein Information: lysine-specific demethylase 3A; testis-specific protein A; jumonji domain containing 1; jumonji domain containing 1A; jumonji domain-containing protein 1A; jumonji C domain-containing histone demethylase 2A; jmjC domain-containing histone demethylation pr
• UniProt Protein Name: Lysine-specific demethylase 3A
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: KDM3A
• UniProt Synonym Gene Names: JHDM2A; JMJD1; JMJD1A; KIAA0742; TSGA
• UniProt Entry Name: KDM3A_HUMAN

NCBI Description

This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Uniprot Description

JMJD1A: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. Belongs to the JHDM2 histone demethylase family.

Protein type: EC 1.14.11.-; Demethylase; Oxidoreductase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function: androgen receptor binding; dioxygenase activity; iron ion binding; transcription factor activity

Biological Process: negative regulation of histone H3-K9 methylation; formaldehyde biosynthetic process; establishment and/or maintenance of chromatin architecture; spermatid nuclear elongation; histone H3-K9 demethylation; hormone-mediated signaling; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter