Rabbit anti-Human KDM3A Polyclonal Antibody | anti-KDM3A antibody

KDM3A (Lysine-specific Demethylase 3A, DKFZp686A24246, DKFZp686P07111, JHDM2A, JHMD2A, JMJD1, Jumonji Domain-containing Protein 1A, JMJD1A, KIAA0742, TSGA) (Biotin)

Cat. Num. AAA6423748

• Gene Names: KDM3A; TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Purified by affinity chromatography.
• Synonyms: KDM3A; Polyclonal Antibody; KDM3A (Lysine-specific Demethylase 3A; DKFZp686A24246; DKFZp686P07111; JHDM2A; JHMD2A; JMJD1; Jumonji Domain-containing Protein 1A; JMJD1A; KIAA0742; TSGA) (Biotin); anti-KDM3A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KDM3A.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-KDM3A antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)
• Application Notes: IF: 1:50-1:100; WB: 1:500-1:2000; IHC: 1:50-1:200; IP: 1:20-1:50; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human KDM3A.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of HeLa cell line, using KDM3A antibody.)

Product Categories/Family for anti-KDM3A antibody

Antibodies; Enzymes; Demethylase

NCBI and Uniprot Product Information

• NCBI GI #: 308153659
• NCBI GeneID: 55818
• UniProt Accession #: Q9Y4C1; Q53S72; Q68D47; Q68UT9; Q6N050; Q8IY08; D6W5M3
• Molecular Weight: 1321
• NCBI Official Full Name: Lysine-specific demethylase 3A
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 3A
• NCBI Official Symbol: KDM3A
• NCBI Official Synonym Symbols: TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A
• NCBI Protein Information: lysine-specific demethylase 3A; testis-specific protein A; jumonji domain containing 1; jumonji domain containing 1A; jumonji domain-containing protein 1A; jumonji C domain-containing histone demethylase 2A; jmjC domain-containing histone demethylation pr
• UniProt Protein Name: Lysine-specific demethylase 3A
• Protein Family: Lysine-specific demethylase
• UniProt Gene Name: KDM3A
• UniProt Synonym Gene Names: JHDM2A; JMJD1; JMJD1A; KIAA0742; TSGA
• UniProt Entry Name: KDM3A_HUMAN

NCBI Description

This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Uniprot Description

JMJD1A: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. Belongs to the JHDM2 histone demethylase family.

Protein type: EC 1.14.11.-; Demethylase; Oxidoreductase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus

Molecular Function: androgen receptor binding; dioxygenase activity; iron ion binding; transcription factor activity

Biological Process: negative regulation of histone H3-K9 methylation; formaldehyde biosynthetic process; establishment and/or maintenance of chromatin architecture; spermatid nuclear elongation; histone H3-K9 demethylation; hormone-mediated signaling; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter