Rabbit anti-Human, Mouse PAN3 Polyclonal Antibody | anti-PAN3 antibody

PAN3 Antibody

Cat. Num. AAA9630080

• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: PAN3; Polyclonal Antibody; PAN3 Antibody; hPan3; PAB dependent poly(A) specific ribonuclease subunit 3; PAB-dependent poly(A)-specific ribonuclease subunit 3; PAB1P dependent poly(A) nuclease; PABP dependent poly(A) nuclease 3; PABP1 dependent poly A specific ribonuclease subunit PAN3; Pan 3; PAN deadenylation complex subunit 3; PAN3 poly(A) specific ribonuclease subunit; PAN3 poly(A) specific ribonuclease subunit homolog; PAN3 polyA specific ribonuclease subunit homolog; PAN3_HUMAN; anti-PAN3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PAN3 Antibody detects endogenous levels of PAN3.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PAN3 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human PAN3.
• Conjugation: Unconjugated
• Similarity: The N-terminal zinc finger binds to poly(A) RNA. Contains a pseudokinase domain. The protein kinase domain is predicted to be catalytically inactive because some of the residues important for catalytic activity are substituted and it lacks the equivalent of the binding site for a peptide substrate. However, it has retained an ATP-binding site and ATP-binding is required for mRNA degradation, stimulating the activity of the PAN2 nuclease in vitro. The nucleotide-binding site is juxtaposed to the RNase active site of PAN2 in the complex and may actually bind nucleosides of a poly(A) RNA rather than ATP, feeding the poly(A)-tail to the active site of the deadenylase and thus increasing the efficiency with which this distributive enzyme degrades oligo(A) RNAs. The pseudokinase domain, the coiled-coil (CC), and C-terminal knob domain (CK) form a structural unit (PKC) that forms an extensive high-affinity interaction surface for PAN2. Belongs to the protein kinase superfamily. PAN3 family.
• Subunit Structure: Homodimer. Forms a heterotrimer with a catalytic subunit PAN2 to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein PABPC1 (via PABC domain), conferring substrate specificity of the enzyme complex. Interacts with the GW182 family proteins TNRC6A, TNRC6B and TNRC6C.
• Function: Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PAN3 antibody

Cytoplasm; P-body; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 281427224
• NCBI GeneID: 255967
• NCBI Accession #: NP_787050.6
• NCBI GenBank Nucleotide #: NM_175854.7
• UniProt Accession #: Q58A45; Q0D2P2; Q5HYG6; Q5T515; Q5T516; Q5TBA0; Q76E13; A0N0X1; A1A4Y8; A1A4Y9; B1ALF1; B7Z3W7
• Molecular Weight: 72,933 Da
• NCBI Official Full Name: PAB-dependent poly(A)-specific ribonuclease subunit PAN3
• NCBI Official Synonym Full Names: PAN3 poly(A) specific ribonuclease subunit
• NCBI Official Symbol: PAN3
• NCBI Protein Information: PAB-dependent poly(A)-specific ribonuclease subunit PAN3; PAB-dependent poly(A)-specific ribonuclease subunit PAN3; PAB1P-dependent poly(A)-nuclease; PABP-dependent poly(A) nuclease 3; PAN deadenylation complex subunit 3; PAN3 poly(A) specific ribonucleas
• UniProt Protein Name: PAB-dependent poly(A)-specific ribonuclease subunit PAN3
• UniProt Gene Name: PAN3
• UniProt Entry Name: PAN3_HUMAN

Uniprot Description

PAN3: Functions in cytoplasmic mRNA decay. As part of the Pan nuclease complex, recruits polyadenylate-binding protein which in turn stimulates PAN2 nuclease activity. Belongs to the protein kinase superfamily. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 13q12.2

Cellular Component: cytosol

Molecular Function: protein binding; poly(A)-specific ribonuclease activity; metal ion binding; transferase activity, transferring phosphorus-containing groups; ATP binding

Biological Process: deadenylation-dependent decapping; poly(A) tail shortening; gene expression; protein targeting; mRNA catabolic process, deadenylation-dependent decay