Rabbit SPIB Polyclonal Antibody | anti-SPIB antibody

SPIB Antibody

Cat. Num. AAA9629849

• Gene Names: SPIB; SPI-B
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SPIB; Polyclonal Antibody; SPIB Antibody; SPI B; Spi B transcription factor; Spib; SPIB_HUMAN; Transcription factor Spi-B; anti-SPIB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: SPIB Antibody detects endogenous levels of SPIB.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-SPIB antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human SPIB.
• Conjugation: Unconjugated
• Similarity: The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes. Belongs to the ETS family.
• Subunit Structure: Can form homotypic interactions. Interacts with IRF4. May also interact with CREBBP, EP300, SPI1/PU.1 related, JUN and TBP.
• Tissue Specificity: Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver.
• Function: Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-SPIB antibody

Nucleus; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 61888836
• NCBI GeneID: 6689
• NCBI Accession #: NP_003112
• NCBI GenBank Nucleotide #: NM_003121.4
• UniProt Accession #: Q01892; Q15359; A8K9C9; B4DUG6
• Molecular Weight: Predicted: 19; 25; 29 kDa; ; Observed: 20 kDa
• NCBI Official Full Name: transcription factor Spi-B isoform 1
• NCBI Official Synonym Full Names: Spi-B transcription factor (Spi-1/PU.1 related)
• NCBI Official Symbol: SPIB
• NCBI Official Synonym Symbols: SPI-B
• NCBI Protein Information: transcription factor Spi-B
• UniProt Protein Name: Transcription factor Spi-B
• Protein Family: Transcription factor
• UniProt Gene Name: SPIB
• UniProt Entry Name: SPIB_HUMAN

NCBI Description

The protein encoded by this gene is a transcriptional activator that binds to the PU-box (5'-GAGGAA-3') and acts as a lymphoid-specific enhancer. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

SPIB: Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: cytoplasm; nucleus

Molecular Function: sequence-specific DNA binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; cell differentiation