Rabbit anti-Human TRAC Polyclonal Antibody | anti-TRAC antibody

TRAC Antibody

Cat. Num. AAA9629784

• Gene Names: TRAC; TRA; IMD7; TCRA; TRCA
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: TRAC; Polyclonal Antibody; TRAC Antibody; T cell antigen receptor alpha polypeptide; T cell receptor alpha; T cell receptor alpha locus; T-cell receptor alpha chain C region; TCRA; TCRD; TRA; TRA@; anti-TRAC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: TRAC Antibody detects endogenous levels of TRAC.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TRAC antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human TRAC.
• Conjugation: Unconjugated
• Similarity: The connecting peptide (CP) domain contributes to the TR-CD3 assembly and signal transduction. The TM domain mediates the interaction with the CD3 subunits.
• Subunit Structure: Alpha-beta TR is a heterodimer composed of an alpha and beta chain; disulfide-linked. The alpha-beta TR is associated with the transmembrane signaling CD3 coreceptor proteins to form the TR-CD3 (TcR or TCR). The assembly of alpha-beta TR heterodimers with CD3 occurs in the endoplasmic reticulum where a single alpha-beta TR heterodimer associates with one CD3D-CD3E heterodimer, one CD3G-CD3E heterodimer and one CD247 homodimer forming a stable octomeric structure. CD3D-CD3E and CD3G-CD3E heterodimers preferentially associate with TR alpha and TR beta chains, respectively. The association of the CD247 homodimer is the last step of TcR assembly in the endoplasmic reticulum and is required for transport to the cell surface.
• Function: Constant region of T cell receptor (TR) alpha chain. Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens. Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-TRAC antibody

Cell membrane; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 135511
• NCBI GeneID: 28755
• UniProt Accession #: P01848
• Molecular Weight: 15,928 Da
• NCBI Official Full Name: T-cell receptor alpha chain C region, partial
• NCBI Official Synonym Full Names: T cell receptor alpha constant
• NCBI Official Symbol: TRAC
• NCBI Official Synonym Symbols: TRA; IMD7; TCRA; TRCA
• UniProt Protein Name: T-cell receptor alpha chain C region
• Protein Family: T-cell receptor
• UniProt Gene Name: TRAC
• UniProt Synonym Gene Names: TCRA
• UniProt Entry Name: TCA_HUMAN