Rabbit anti-Human TIFA Polyclonal Antibody | anti-TIFA antibody

TIFA Antibody

Cat. Num. AAA9629744

• Gene Names: TIFA; T2BP; T6BP; TIFAA
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: TIFA; Polyclonal Antibody; TIFA Antibody; anti-TIFA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: TIFA Antibody detects endogenous levels of TIFA.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TIFA antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human TIFA.
• Conjugation: Unconjugated
• Similarity: The FHA domain recognizes and binds phosphorylated Thr-9, promoting homooligomerization and subsequent activation of NF-kappa-B. Belongs to the TIFA family.
• Post Translational Modifications: Phosphorylated at Thr-9 following detection of ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose) by ALPK1. Phosphorylation at Thr-9 by ALPK1 leads to the formation of an intermolecular binding between the FHA domain and phosphorylated Thr-9, promoting TIFA oligomerization and TIFA-mediated NF-kappa-B activation.
• Subunit Structure: Homooligomer; homooligomerizes following phosphorylation at Thr-9. Interacts with IRAK1, TRAF2 and TRAF6. Interacts with TIFAB; binding to TIFAB inhibits TRAF6 activation, possibly by inducing a conformational change in TIFA. Interacts with ZCCHC11; binding to ZCCHC11 suppresses the TRAF6-dependent activation of NF-kappa-B.
• Function: Adapter molecule that plays a key role in the activation of proinflammatory NF-kappa-B signaling following detection of bacterial pathogen-associated molecular pattern metabolites (PAMPs). Promotes activation of an innate immune response by inducing the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism. TIFA-dependent innate immune response is triggered by ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria: ADP-Heptose is recognized by ALPK1, which phosphorylates TIFA at Thr-9, leading to TIFA homooligomerization and subsequent activation of proinflammatory NF-kappa-B signaling.
• Note: Colocalizes with lysosomal marker LAMP2 following homooligomerization and subsequent activation.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-TIFA antibody

Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 33563370
• NCBI GeneID: 92610
• NCBI Accession #: NP_443096.1
• NCBI GenBank Nucleotide #: NM_052864.2
• UniProt Accession #: Q96CG3
• Molecular Weight: 21,445 Da
• NCBI Official Full Name: TRAF-interacting protein with FHA domain-containing protein A
• NCBI Official Synonym Full Names: TRAF-interacting protein with forkhead-associated domain
• NCBI Official Symbol: TIFA
• NCBI Official Synonym Symbols: T2BP; T6BP; TIFAA
• NCBI Protein Information: TRAF-interacting protein with FHA domain-containing protein A; TRAF-interacting protein with a forkhead-associated domain; TRAF2 binding protein; TRAF2-binding protein; TRAF6 binding protein; putative MAPK-activating protein PM14; putative NF-kappa-B-acti
• UniProt Protein Name: TRAF-interacting protein with FHA domain-containing protein A
• Protein Family: TRAF-interacting protein with FHA domain-containing protein
• UniProt Gene Name: TIFA
• UniProt Synonym Gene Names: T2BP
• UniProt Entry Name: TIFA_HUMAN

Uniprot Description

TIFA: Adapter protein which mediates the IRAK1 and TRAF6 interaction following IL-1 stimulation, resulting in the downstream activation of NF-kappa-B and AP-1 pathways. Induces the oligomerization and polyubiquitination of TRAF6, which leads to the activation of TAK1 and IKK through a proteasome-independent mechanism.

Chromosomal Location of Human Ortholog: 4q25

Molecular Function: protein binding

Biological Process: I-kappaB kinase/NF-kappaB cascade