Rabbit anti-Human, Mouse SMCR8 Polyclonal Antibody | anti-SMCR8 antibody
SMCR8 Antibody
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SMCR8; Polyclonal Antibody; SMCR8 Antibody; 2310076G09Rik; AI642055; D030073L15Rik; RGD1564621; RP23-278I21.6; SMCR8_HUMAN; Smith-Magenis syndrome chromosomal region candidate gene 8 protein; anti-SMCR8 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
SMCR8 Antibody detects endogenous levels of total SMCR8.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-SMCR8 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from Human MYOCD.
Conjugation
Unconjugated
Similarity
Belongs to the SMCR8 family.
Post Translational Modifications
Phosphorylation by TBK1 is required to promote autophagosome maturation. Phosphorylated by ULK1.
Subunit Structure
Interacts with C9orf72; the interaction is direct. Component of the C9orf72-SMCR8 complex, at least composed of C9orf72, SMCR8 and WDR41. The C9orf72-SMCR8 complex associates with the ATG1/ULK1 kinase complex.
Tissue Specificity
Expressed in all tissues tested.
Function
Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy. In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation. The C9orf72-SMCR8 complex also acts as a negative regulator of autophagy initiation by interacting with the ATG1/ULK1 kinase complex and inhibiting its protein kinase activity. Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates. In addition to its activity in the cytoplasm within the C9orf72-SMCR8 complex, SMCR8 also localizes in the nucleus, where it associates with chromatin and negatively regulates expression of suppresses ULK1 and WIPI2 genes.
Note
Localizes mainly in the cytoplasm.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
105,022 Da
NCBI Official Full Name
Smith-Magenis syndrome chromosomal region candidate gene 8 protein
NCBI Official Synonym Full Names
Smith-Magenis syndrome chromosome region, candidate 8
NCBI Official Symbol
SMCR8
NCBI Protein Information
Smith-Magenis syndrome chromosomal region candidate gene 8 protein
UniProt Protein Name
Smith-Magenis syndrome chromosomal region candidate gene 8 protein
UniProt Gene Name
SMCR8
UniProt Entry Name
SMCR8_HUMAN
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Product Notes
The SMCR8 smcr8 (Catalog #AAA9629667) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMCR8 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SMCR8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the SMCR8 smcr8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SMCR8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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