Rabbit anti-Human, Mouse GGA1 Polyclonal Antibody | anti-GGA1 antibody

GGA1 Antibody

Cat. Num. AAA9629627

• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: GGA1; Polyclonal Antibody; GGA1 Antibody; 4930406E12Rik; ADP ribosylation factor binding protein 1; ADP ribosylation factor binding protein GGA1; ADP-ribosylation factor-binding protein GGA1; ARF binding protein 1; ARF-binding protein 1; Gamma adaptin related protein 1; gamma ear-containing; Gamma-adaptin-related protein 1; GGA 1; GGA1 protein; GGA1_HUMAN; Golgi associated gamma adaptin ear containing ARF; Golgi associated gamma adaptin ear containing ARF binding protein 1; Golgi localized gamma ear containing ARF binding protein 1; Golgi-localized; OTTHUMP00000028975; OTTHUMP00000042200; anti-GGA1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: GGA1 Antibody detects endogenous levels of total GGA1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GGA1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The VHS domain functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines (DXXLL motif). The GAT domain is responsible for interaction with ARF-GTP, UBC and RABEP1. Required for recruitment to the TGN it prevents ARF-GTP hydrolysis. The unstructured hinge region contains clathrin-binding but no autoinhibitory (DXXLL) motifs. The GAE domain binds accessory proteins regulating GGAs function. Belongs to the GGA protein family.
• Post Translational Modifications: Phosphorylated by CK2 and dephosphorylated by PP2A. Phosphorylation of GGA1 allows the internal DXXLL motif to bind the VHS domain and to inhibit the recognition of cargo signals.Ubiquitinated.
• Subunit Structure: Monomer (Probable). Interacts with GGA2 and GGA3. Binds to clathrin and activated ARFs, including ARF1, ARF5 and ARF6. Interacts with RABEP1 and RABGEF1. Interacts with the type-I membrane proteins LRP3, M6PR/CD-MPR and IGF2R/CI-MPR. Interacts (via N-terminal VHS domain) with SORL1/sorLA and SORT1 (via C-terminal cytosolic domain). Interacts with EPN4. Binds CCDC91, P200 and SYNRG. Interacts with NECAP1, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and UBC. Interacts with RNF11. Interacts (via VHS domain) with BACE1 (via DXXLL motif); the interaction highly increases when BACE1 is phosphorylated at 'Ser-498'. Interacts with CNST (By similarity). Interacts with ADRA2B. Interacts with ARL3; the interaction recruits, in collaboration with RABEP1, PKD1:PKD2 complex to trans-Golgi network and is required for ciliary targeting (By similarity).
• Tissue Specificity: Ubiquitously expressed.
• Function: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Required for targeting PKD1:PKD2 complex from the trans-Golgi network to the cilium membrane (By similarity). Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-GGA1 antibody

Golgi apparatus; trans-Golgi network membrane; Peripheral membrane protein; Endosome membrane; Early endosome membrane; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 14548066
• NCBI GeneID: 26088
• UniProt Accession #: Q9UJY5; Q5TG07; Q86YA9; Q8NCS6; Q9BW94; Q9UG00; Q9UGW0; Q9UGW1; A8K3D3; B0QYR7
• Molecular Weight: 9,969 Da
• NCBI Official Full Name: ADP-ribosylation factor-binding protein GGA1
• NCBI Official Synonym Full Names: golgi-associated, gamma adaptin ear containing, ARF binding protein 1
• NCBI Official Symbol: GGA1
• NCBI Protein Information: ADP-ribosylation factor-binding protein GGA1; gamma-adaptin related protein 1; gamma-adaptin-related protein 1; ADP-ribosylation factor binding protein 1; golgi-localized, gamma ear-containing, ARF-binding protein 1; golgi associated, gamma adaptin ear co
• UniProt Protein Name: ADP-ribosylation factor-binding protein GGA1
• Protein Family: ADP-ribosylation factor-binding protein
• UniProt Gene Name: GGA1
• UniProt Entry Name: GGA1_HUMAN

NCBI Description

This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GGA1: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Belongs to the GGA protein family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 22q13.31

Cellular Component: nucleoplasm; Golgi apparatus; clathrin adaptor complex; intracellular membrane-bound organelle; membrane; endosome membrane

Molecular Function: protein binding

Biological Process: vesicle-mediated transport; intracellular protein transport; positive regulation of protein catabolic process