Rabbit PARP14 Polyclonal Antibody | anti-PARP14 antibody
PARP14 Antibody
Gene Names
PARP14; BAL2; ARTD8; pART8; PARP-14
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
PARP14; Polyclonal Antibody; PARP14 Antibody; ADP-ribosyltransferase diphtheria toxin-like 8; ARTD8; B aggressive lymphoma protein 2; BAL2; Collaborator of STAT6; KIAA1268; PAR14_HUMAN; PARP 14; PARP-14; pART8; Poly (ADP ribose) polymerase family member 14; Poly [ADP-ribose] polymerase 14; anti-PARP14 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
PARP14 Antibody detects endogenous levels of total PARP14.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PARP14 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from Human MYOCD.
Conjugation
Unconjugated
Post Translational Modifications
Auto-ADP-ribosylated.
Subunit Structure
Interacts with STAT6 (By similarity). Interacts with PARP10. Interacts with PARP9 in IFNG-stimulated macrophages; the interaction prevents PARP14-mediated STAT1 and STAT6 ADP-riboslylation.
Tissue Specificity
Expressed in macrophages.
Function
ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins. In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation. Has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', thus decreasing STAT1 phosphorylation which negatively regulates pro-inflammatory cytokine production in macrophages in response to IFNG stimulation. However, the role of ADP-ribosylation in the prevention of STAT1 phosphorylation has been called into question and it has been suggested that the inhibition of phosphorylation may be the result of sumoylation of STAT1 'Lys-703'. Mono-ADP-ribosylates STAT6; enhancing STAT6-dependent transcription. In macrophages, positively regulates MRC1 expression in response to IL4 stimulation by promoting STAT6 phosphorylation. Mono-ADP-ribosylates PARP9.
Note
In steady state splenocytes the protein is mostly nuclear (By similarity). A minor proportion is detected in the cytoplasm (By similarity). In macrophages, mainly localizes to the cytoplasm (PubMed:27796300).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
89,790 Da
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly(ADP-ribose) polymerase family member 14
NCBI Official Symbol
PARP14
NCBI Official Synonym Symbols
BAL2; ARTD8; pART8; PARP-14
NCBI Protein Information
poly [ADP-ribose] polymerase 14
UniProt Protein Name
Poly [ADP-ribose] polymerase 14
Protein Family
UniProt Gene Name
PARP14
UniProt Synonym Gene Names
BAL2; KIAA1268; PARP-14; ARTD8
NCBI Description
This gene encodes a member of the poly(ADP-ribose) polymerase (PARP) protein family. The encoded anti-apoptotic protein may regulate aerobic glycolysis and promote survival of cancer cells. Increased expression of this gene has been reported in a variety of tumor types. [provided by RefSeq, Jul 2016]
Uniprot Description
PARP14: Enhances STAT6-dependent transcription. Has ADP-ribosyltransferase activity. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.4.2.30; Transcription, coactivator/corepressor; Transferase
Chromosomal Location of Human Ortholog: 3q21.1
Cellular Component: cytoplasm; cytosol; membrane; nucleus
Molecular Function: enzyme binding; NAD+ ADP-ribosyltransferase activity; protein binding; RNA binding
Biological Process: innate immune response; negative regulation of gene expression; negative regulation of tyrosine phosphorylation of STAT protein; positive regulation of interleukin-4-mediated signaling pathway; positive regulation of tyrosine phosphorylation of STAT protein; protein ADP-ribosylation; regulation of transcription, DNA-templated; transcription, DNA-dependent
Research Articles on PARP14
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Product Notes
The PARP14 parp14 (Catalog #AAA9629462) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PARP14 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PARP14 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the PARP14 parp14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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