Rabbit anti-Human, Mouse LRDD Polyclonal Antibody | anti-PIDD1 antibody

LRDD Antibody

Cat. Num. AAA9629366

• Gene Names: PIDD1; LRDD; PIDD
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: LRDD; Polyclonal Antibody; LRDD Antibody; DKFZp434D229; Leucine rich repeats and death domain containing; Leucine-rich repeats and death domain containing; MGC16925; p53-induced protein with a death domain; PIDD; anti-PIDD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: LRDD Antibody detects endogenous levels of total LRDD.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PIDD1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The Death domain mediates the interaction with CRADD and the formation of a complex composed of 5 PIDD1 and 7 CRADD proteins which in turn recruit 7 CASP2 to form the PIDDosome. The LRR repeat-containing domain has a regulatory activity, being autoinhibitory for the activation of NF-kappa-B.
• Post Translational Modifications: Undergoes autoproteolytic processing whose extent either directs cells towards survival or apoptotic pathways. Autoproteolytically cleaved into two main fragments PIDD-N and PIDD-C. PIDD-C can be further processed into PIDD-CC, a processing which is enhanced by DNA damage. The cleavage producing PIDD-C is required for translocation of PIDD1 to the nucleus upon DNA damage and activation of NF-kappa-B. PIDD-CC mediates the interaction with CRADD and the cleavage producing PIDD-CC is required for the activation of CASP2. PIDD-N remains associated with PIDD-C and PIDD-CC after cleavage.
• Subunit Structure: Forms a complex named the PIDDosome with CASP2 and CRADD. Forms a complex with IKBKG and RIPK1. Interacts with FADD and MADD.
• Tissue Specificity: Ubiquitous.
• Function: Component of the DNA damage/stress response pathway that functions downstream of p53/TP53 and can either promote cell survival or apoptosis. Associated with CRADD and the CASP2 caspase, it forms the PIDDosome a complex that activates CASP2 and triggers apoptosis. Associated with IKBKG and RIPK1, it enhances sumoylation and ubiquitination of IKBKG which is important for activation of the transcription factor NF-kappa-B.
• Note: Enriched in the nucleus upon DNA damage.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-PIDD1 antibody

Cytoplasm; Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 116242715
• NCBI GeneID: 55367
• UniProt Accession #: Q9HB75; Q59FD1; Q59H10; Q59HC7; Q7Z4P8; Q8NC89; Q8NDL2; Q96C25; Q9NRE6
• Molecular Weight: 910
• NCBI Official Full Name: p53-induced death domain-containing protein 1
• NCBI Official Synonym Full Names: p53-induced death domain protein 1
• NCBI Official Symbol: PIDD1
• NCBI Official Synonym Symbols: LRDD; PIDD
• NCBI Protein Information: p53-induced death domain-containing protein 1; p53-induced protein with a death domain; leucine-rich repeats and death domain containing; leucine-rich repeat and death domain-containing protein
• UniProt Protein Name: p53-induced death domain-containing protein 1
• Protein Family: p53-induced death domain-containing protein
• UniProt Gene Name: PIDD1
• UniProt Synonym Gene Names: LRDD; PIDD
• UniProt Entry Name: PIDD1_HUMAN

NCBI Description

The protein encoded by this gene contains a leucine-rich repeat and a death domain. This protein has been shown to interact with other death domain proteins, such as Fas (TNFRSF6)-associated via death domain (FADD) and MAP-kinase activating death domain-containing protein (MADD), and thus may function as an adaptor protein in cell death-related signaling processes. The expression of the mouse counterpart of this gene has been found to be positively regulated by the tumor suppressor p53 and to induce cell apoptosis in response to DNA damage, which suggests a role for this gene as an effector of p53-dependent apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2010]

Uniprot Description

PIDD: Promotes apoptosis downstream of the tumor suppressor as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. Associates with NEMO/IKBKG and RIP1 and enhances sumoylation and ubiquitination of NEMO/IKBKG which is important for activation of the transcription factor NF-kappa-B. Associates with CASP2/caspase-2 and CRADD/RAIDD, and induces activation of CASP2 which an important regulator in apoptotic pathways. 7 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: Golgi apparatus; cytoplasm; nucleus

Molecular Function: protein binding; death receptor binding

Biological Process: caspase activation; positive regulation of apoptosis; signal transduction; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; response to DNA damage stimulus; negative regulation of apoptosis; activation of NF-kappaB transcription factor