Mouse Claudin 18.2 Monoclonal Antibody | anti-CLDN18 antibody

Claudin 18.2 Mouse monoclonal antibody

Cat. Num. AAA9629041

• Gene Names: CLDN18; SFTA5; SFTPJ; DKFZp564B2062
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity-chromatography.
• Synonyms: Claudin 18.2; Monoclonal Antibody; Claudin 18.2 Mouse monoclonal antibody; Claudin 18; Claudin-18; CLD18_HUMAN; CLDN18; SFTA5; SFTPJ; anti-CLDN18 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Specificity: Claudin 18.2 Mouse monoclonal antibody detects endogenous levels of total Claudin 18.2.
• Purity/Purification: Affinity-chromatography.
• Form/Format: Liquid. Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CLDN18 antibody: Western Blot (WB)
• Application Notes: WB: 1:2000-5000
• Immunogen: A synthesized peptide derived from Human CLDN18.2.
• Conjugation: Unconjugated
• Similarity: Belongs to the claudin family.
• Tissue Specificity: Isoform A1: Expression is restricted to the lung. Isoform A2: Expression is restricted to the stomach mucosa where it is predominantly observed in the epithelial cells of the pit region and the base of the gastric glands including exocrine and endocrine cells (at protein level).
• Function: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
• Note: Localizes to tight junctions in epithelial cells.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis on various lysates using Claudin 18.2 Mouse monoclonal antibody.lane1 rat stomach with blocking peptide, lane2 rat stomach, lane3 mouse stomach.observed:26kD.)

Related Product Information for anti-CLDN18 antibody

Claudin-18 (CLDN18) is a member of a large family of four-span transmembrane proteins, Claudins, which are the essential components of the mammalian tight junctions (TJs) in the epithelial cells. Claudin-18 has two splice variants, 18.1 and 18.2. While CLDN18.1 is specifically expressed in the lung tissue, CLDN18.2 expression in normal tissue is more restricted and is only detected in small patches of stomach mucosal. CLDN18.2 expression is elevated in many types of epithelial cancers including stomach, esophagus, pancreatic and ovarian cancers. The expression of CLDN18.2 is not only detected in primary tumors, but also in the metastatic sites. Therefore, CLDN18.2 is an ideal target for monoclonal antibody-based cancer therapies. CLDN18.2-specific antibodies are difficult to generate due to its high homology to CLDN18.1. There are only 7-8 amino acid differences between these two splice variants in the first extracellular loop. Using a combination of DNA, protein, and transfected cells as immunogens, coupled with high-throughput FACS-based hybridoma screening approach, we have generated a panel of mouse monoclonal antibodies highly specific for CLDN18.2, CLDN18.2 expression in normal tissue is more restricted and is only detected in small patches of stomach mucosal. CLDN18.2 expression is elevated in many types of epithelial cancers including stomach, esophagus, pancreatic and ovarian cancers.

Product Categories/Family for anti-CLDN18 antibody

Cell junction; Tight junction; Cell membrane; Multi-pass membrane protein; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 50345279
• NCBI GeneID: 51208
• NCBI Accession #: NP_001002026.1
• NCBI GenBank Nucleotide #: NM_001002026.2
• UniProt Accession #: P56856; Q96PH4; A5PL21
• Molecular Weight: 27,856 Da
• NCBI Official Full Name: claudin-18 isoform 2
• NCBI Official Synonym Full Names: claudin 18
• NCBI Official Symbol: CLDN18
• NCBI Official Synonym Symbols: SFTA5; SFTPJ; DKFZp564B2062
• NCBI Protein Information: claudin-18; OTTHUMP00000216399; OTTHUMP00000216400; surfactant associated 5; surfactant associated protein J; surfactant, pulmonary associated protein J
• UniProt Protein Name: Claudin-18
• Protein Family: Claudin
• UniProt Gene Name: CLDN18
• UniProt Entry Name: CLD18_HUMAN

NCBI Description

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]

Uniprot Description

Claudin-18: a multi-pass membrane protein with calcium-independent cell-adhesion activity that plays a major role in the formation of tight junctions. Belongs to the claudin family. Two isoforms of the human protein are produced by alternative splicing. Isoform 2 is a specific cell lineage marker. Its expression in normal tissues is restricted to differentiated epithelial cells of the gastric mucosa; it is absent from the gastric stem cells. Isoform 2 is expressed in a significant proportion of primary gastric cancers and metastases, as well as in subtypes of pancreatic, esophageal, ovarian, and lung tumors.

Protein type: Cell adhesion; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q22.3

Cellular Component: tight junction; integral to membrane; plasma membrane

Molecular Function: identical protein binding; structural molecule activity

Biological Process: intercellular junction assembly and maintenance; calcium-independent cell-cell adhesion