Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Stomach. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Mouse anti-Human Claudin 18 Monoclonal Antibody | anti-CLDN18 antibody
Claudin 18 (5F3) Mouse mAb
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Stomach. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Stomach. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Stomach. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded Stomach. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010]
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
Uniprot Description
Claudin-18: a multi-pass membrane protein with calcium-independent cell-adhesion activity that plays a major role in the formation of tight junctions. Belongs to the claudin family. Two isoforms of the human protein are produced by alternative splicing. Isoform 2 is a specific cell lineage marker. Its expression in normal tissues is restricted to differentiated epithelial cells of the gastric mucosa; it is absent from the gastric stem cells. Isoform 2 is expressed in a significant proportion of primary gastric cancers and metastases, as well as in subtypes of pancreatic, esophageal, ovarian, and lung tumors.
Protein type: Cell adhesion; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q22.3
Cellular Component: tight junction; integral to membrane; plasma membrane
Molecular Function: identical protein binding; structural molecule activity
Biological Process: intercellular junction assembly and maintenance; calcium-independent cell-cell adhesion
Research Articles on CLDN18
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Product Notes
The CLDN18 cldn18 (Catalog #AAA4753953) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Claudin 18 (5F3) Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Claudin 18 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:100-1:500. Researchers should empirically determine the suitability of the CLDN18 cldn18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Claudin 18, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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