SLC3A2 blocking peptide

SLC3A2 Blocking Peptide

Cat. Num. AAA9626816

• Gene Names: SLC3A2; 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: SLC3A2; SLC3A2 Blocking Peptide; 4F2; 4F2 cell surface antigen heavy chain; 4F2 cell-surface antigen heavy chain; 4F2 heavy chain; 4F2 heavy chain antigen; 4F2_HUMAN; 4F2hc; 4T2HC; Antigen defined by monoclonal antibody 4F2 heavy chain; Antigen identified by monoclonal antibodies 4F2 TRA1.10 TROP4 and T43; CD 98; CD98; CD98 antigen; CD98 heavy chain; CD98HC; Heavy chain; Lymphocyte activation antigen 4F2 large subunit; MDU 1; MDU1; Monoclonal antibody 44D7; NACAE; Slc3a2; Solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2; Solute carrier family 3 member 2; SLC3A2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for SLC3A2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SLC3A2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 61744477
• NCBI GeneID: 6520
• NCBI Accession #: NP_001012680.1
• NCBI GenBank Nucleotide #: NM_001012662.2
• UniProt Accession #: P08195; Q13543
• Molecular Weight: 71,123 Da
• NCBI Official Full Name: 4F2 cell-surface antigen heavy chain isoform b
• NCBI Official Synonym Full Names: solute carrier family 3 (amino acid transporter heavy chain), member 2
• NCBI Official Symbol: SLC3A2
• NCBI Official Synonym Symbols: 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC
• NCBI Protein Information: 4F2 cell-surface antigen heavy chain; CD98 heavy chain; antigen defined by monoclonal antibody 4F2, heavy chain; antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43; lymphocyte activation antigen 4F2 large subunit; monoclonal antibody
• UniProt Protein Name: 4F2 cell-surface antigen heavy chain
• Protein Family: 4F2 cell-surface antigen
• UniProt Gene Name: SLC3A2
• UniProt Synonym Gene Names: MDU1; 4F2hc

NCBI Description

This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized. [provided by RefSeq, Nov 2010]

Uniprot Description

Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. When associated with LAPTM4B, recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (PubMed:25998567).