ATPAF2 blocking peptide

ATPAF2 Blocking Peptide

Cat. Num. AAA9623978

• Gene Names: ATPAF2; ATP12; ATP12p; LP3663; MC5DN1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: ATPAF2; ATPAF2 Blocking Peptide; ATP synthase mitochondrial F1 complex assembly factor 2; ATP12; ATP12 homolog; ATP12p; LP3663; MC5DN1; ATPAF2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for ATPAF2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ATPAF2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 21735485
• NCBI GeneID: 91647
• NCBI Accession #: NP_663729.1
• NCBI GenBank Nucleotide #: NM_145691.3
• UniProt Accession #: Q8N5M1; Q6XYC7; A6NDE5; A8K2J2
• Molecular Weight: 32,772 Da
• NCBI Official Full Name: ATP synthase mitochondrial F1 complex assembly factor 2
• NCBI Official Synonym Full Names: ATP synthase mitochondrial F1 complex assembly factor 2
• NCBI Official Symbol: ATPAF2
• NCBI Official Synonym Symbols: ATP12; ATP12p; LP3663; MC5DN1
• NCBI Protein Information: ATP synthase mitochondrial F1 complex assembly factor 2; ATP12 homolog
• UniProt Protein Name: ATP synthase mitochondrial F1 complex assembly factor 2
• Protein Family: ATP synthase mitochondrial F1 complex assembly factor
• UniProt Gene Name: ATPAF2
• UniProt Synonym Gene Names: ATP12
• UniProt Entry Name: ATPF2_HUMAN

NCBI Description

This gene encodes an assembly factor for the F(1) component of the mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. This gene is located within the Smith-Magenis syndrome region on chromosome 17. An alternatively spliced transcript variant has been described, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

ATPAF2: May play a role in the assembly of the F1 component of the mitochondrial ATP synthase (ATPase). Defects in ATPAF2 are a cause of mitochondrial complex V deficiency nuclear type 1 (MC5DN1). A mitochondrial disorder with heterogeneous clinical manifestations including dysmorphic features, psychomotor retardation, hypotonia, growth retardation, cardiomyopathy, enlarged liver, hypoplastic kidneys and elevated lactate levels in urine, plasma and cerebrospinal fluid. Belongs to the ATP12 family.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: mitochondrion; nuclear speck

Molecular Function: protein binding

Biological Process: proton-transporting ATP synthase complex assembly

Disease: Mitochondrial Complex V (atp Synthase) Deficiency, Nuclear Type 1