UBF1 blocking peptide

UBF1 Blocking Peptide

Cat. Num. AAA9623282

• Gene Names: UBTF; UBF; UBF1; UBF-1; NOR-90
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: UBF1; UBF1 Blocking Peptide; 90 kDa nucleolus organizer region autoantigen; Autoantigen NOR-90; NOR 90; Nucleolar transcription factor 1; UBF 1; UBF; UBF-1; UBF1_HUMAN; Ubtf; Upstream binding factor 1; upstream binding transcription factor; RNA polymerase I; Upstream-binding factor 1; UBF1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for UBF1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for UBF1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 115529451
• NCBI GeneID: 7343
• NCBI Accession #: NP_001070151.1
• NCBI GenBank Nucleotide #: NM_001076683.1
• UniProt Accession #: P17480; A8K6R8
• Molecular Weight: 89,406 Da
• NCBI Official Full Name: nucleolar transcription factor 1 isoform b
• NCBI Official Synonym Full Names: upstream binding transcription factor, RNA polymerase I
• NCBI Official Symbol: UBTF
• NCBI Official Synonym Symbols: UBF; UBF1; UBF-1; NOR-90
• NCBI Protein Information: nucleolar transcription factor 1; autoantigen NOR-90; 90-kDa nucleolus organizer region autoantigen
• UniProt Protein Name: Nucleolar transcription factor 1
• UniProt Gene Name: UBTF
• UniProt Synonym Gene Names: UBF; UBF1; UBF-1
• UniProt Entry Name: UBF1_HUMAN

NCBI Description

This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011]

Uniprot Description

UBF: a transcription factor that recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the species-specific factor SL1. Activate RNA polymerase II-regulated, beta-catenin-responsive promoters. Interacts with TAF1, a factor involved in the transcription of cell cycle and growth regulatory genes. It binds specifically to the upstream control element. Two splice variant isoforms have been described.

Protein type: Nucleolus; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: nucleoplasm; nucleolus

Molecular Function: protein binding; DNA binding; chromatin binding

Biological Process: chromatin remodeling; regulation of transcription from RNA polymerase I promoter; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; positive regulation of transcription from RNA polymerase I promoter