ATP6V1D blocking peptide

ATP6V1D Blocking Peptide

Cat. Num. AAA9622517

• Gene Names: ATP6V1D; VATD; VMA8; ATP6M
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: ATP6V1D; ATP6V1D Blocking Peptide; ATP6M; V-ATPase 28 kDa accessory protein; V-ATPase subunit D; V-type proton ATPase subunit D; Vacuolar ATP synthase subunit D; Vacuolar proton pump subunit D; VATD; VMA8; ATP6V1D blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for ATP6V1D blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ATP6V1D blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 7706757
• NCBI GeneID: 51382
• NCBI Accession #: NP_057078.1
• NCBI GenBank Nucleotide #: NM_015994.3
• UniProt Accession #: Q9Y5K8; Q9Y688; B2RE33
• Molecular Weight: 28,263 Da
• NCBI Official Full Name: V-type proton ATPase subunit D
• NCBI Official Synonym Full Names: ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
• NCBI Official Symbol: ATP6V1D
• NCBI Official Synonym Symbols: VATD; VMA8; ATP6M
• NCBI Protein Information: V-type proton ATPase subunit D; V-ATPase D subunit; V-ATPase subunit D; vacuolar H-ATPase subunit D; vacuolar proton pump D subunit; vacuolar proton pump subunit D; vacuolar ATP synthase subunit D; vacuolar proton-ATPase subunit D; V-ATPase 28 kDa accesso
• UniProt Protein Name: V-type proton ATPase subunit D
• UniProt Gene Name: ATP6V1D
• UniProt Synonym Gene Names: ATP6M; VATD; V-ATPase subunit D
• UniProt Entry Name: VATD_HUMAN

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes the V1 domain D subunit protein. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6V1D: Subunit of the peripheral V1 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. Belongs to the V-ATPase D subunit family.

Protein type: EC 3.6.3.14; Hydrolase; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 14q23-q24.2

Cellular Component: centrosome; membrane; lysosomal membrane; cytosol; cilium

Molecular Function: protein binding; ATPase activity, coupled to transmembrane movement of substances

Biological Process: interaction with host; proton transport; cellular iron ion homeostasis; insulin receptor signaling pathway; cilium biogenesis; transferrin transport; transmembrane transport