SLC6A3 blocking peptide

SLC6A3 Blocking Peptide

Cat. Num. AAA9622499

• Gene Names: SLC6A3; DAT; DAT1; PKDYS
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: SLC6A3; SLC6A3 Blocking Peptide; DA transporter; DAT 1; DAT; DAT1; Dopamine transporter 1; Dopamine transporter; PKDYS; SC6A3_HUMAN; Sodium dependent dopamine transporter; Sodium-dependent dopamine transporter; Solute carrier family 6 (neurotransmitter transporter dopamine); member 3; Solute carrier family 6 (neurotransmitter transporter); Solute carrier family 6 member 3; Variable number tandem repeat (VNTR); SLC6A3 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for SLC6A3 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for SLC6A3 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4507041
• NCBI GeneID: 6531
• NCBI Accession #: NP_001035.1
• NCBI GenBank Nucleotide #: NM_001044.4
• UniProt Accession #: Q01959; Q14996; A2RUN4
• Molecular Weight: 68,495 Da
• NCBI Official Full Name: sodium-dependent dopamine transporter
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
• NCBI Official Symbol: SLC6A3
• NCBI Official Synonym Symbols: DAT; DAT1; PKDYS
• NCBI Protein Information: sodium-dependent dopamine transporter; DA transporter; solute carrier family 6 member 3
• UniProt Protein Name: Sodium-dependent dopamine transporter
• UniProt Gene Name: SLC6A3
• UniProt Synonym Gene Names: DAT1; DA transporter; DAT
• UniProt Entry Name: SC6A3_HUMAN

NCBI Description

This gene encodes a dopamine transporter which is a member of the sodium- and chloride-dependent neurotransmitter transporter family. The 3' UTR of this gene contains a 40 bp tandem repeat, referred to as a variable number tandem repeat or VNTR, which can be present in 3 to 11 copies. Variation in the number of repeats is associated with idiopathic epilepsy, attention-deficit hyperactivity disorder, dependence on alcohol and cocaine, susceptibility to Parkinson disease and protection against nicotine dependence.[provided by RefSeq, Nov 2009]

Uniprot Description

DAT: Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals. Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI). It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs. Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.

Protein type: Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15.3

Cellular Component: cell soma; axon; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane

Molecular Function: protein binding; protease binding; dopamine binding; monoamine transmembrane transporter activity; protein complex binding; protein N-terminus binding; dopamine:sodium symporter activity; drug binding; dopamine transmembrane transporter activity; receptor binding

Biological Process: lactation; response to drug; response to nicotine; dopamine transport; response to cAMP; prepulse inhibition; dopamine catabolic process; regulation of dopamine metabolic process; monoamine transport; sensory perception of smell; locomotory behavior; dopamine biosynthetic process; positive regulation of multicellular organism growth; response to cocaine; synaptic transmission; response to ethanol; neurotransmitter transport; adenohypophysis development; response to iron ion; transmembrane transport; aging; neurotransmitter biosynthetic process

Disease: Parkinsonism-dystonia, Infantile; Tobacco Addiction, Susceptibility To