LGI1 blocking peptide

Lgi1 Blocking Peptide

Cat. Num. AAA9621988

• Gene Names: LGI1; EPT; ETL1; ADLTE; ADPAEF; ADPEAF; IB1099; EPITEMPIN
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: Lgi1; Lgi1 Blocking Peptide; ADLTE; ADPAEF; ADPEAF; Epitempin 1; EPITEMPIN; Epitempin-1; EPT; ETL1; IB1099; leucine rich glioma inactivated 1; Leucine rich glioma-inactivated protein 1; Leucine-rich glioma-inactivated protein 1; LGI1; LGI1_HUMAN; OTTHUMP00000020121; OTTHUMP00000020122; LGI1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for LGI1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for LGI1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 37182888
• NCBI GeneID: 9211
• UniProt Accession #: O95970; Q5W001; Q5W002; Q8NI23; Q96LF5; A8K0Z1; B4E1S0
• Molecular Weight: 58,302 Da
• NCBI Official Full Name: LGI1
• NCBI Official Synonym Full Names: leucine-rich, glioma inactivated 1
• NCBI Official Symbol: LGI1
• NCBI Official Synonym Symbols: EPT; ETL1; ADLTE; ADPAEF; ADPEAF; IB1099; EPITEMPIN
• NCBI Protein Information: leucine-rich glioma-inactivated protein 1; epitempin-1
• UniProt Protein Name: Leucine-rich glioma-inactivated protein 1
• Protein Family: Leucine-rich glioma-inactivated protein
• UniProt Gene Name: LGI1
• UniProt Synonym Gene Names: EPT
• UniProt Entry Name: LGI1_HUMAN

NCBI Description

This gene is rearranged as a result of translocations in glioblastoma cell lines. The protein contains a hydrophobic segment representing a putative transmembrane domain with the amino terminus located outside the cell. It also contains leucine-rich repeats with conserved cysteine-rich flanking sequences. This gene is predominantly expressed in neural tissues and its expression is reduced in low grade brain tumors and significantly reduced or absent in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. [provided by RefSeq, Jul 2008]

Uniprot Description

LGI1: Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors. Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival. Defects in LGI1 are the cause of epilepsy, familial temporal lobe, type 1 (ETL1). A focal form of epilepsy characterized by recurrent seizures that arise from foci within the temporal lobe. Seizures are usually accompanied by sensory symptoms, most often auditory in nature. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: extracellular space; extracellular region; synapse; cell junction

Molecular Function: protein binding; receptor binding

Biological Process: cell proliferation; nervous system development; axon guidance; positive regulation of cell growth; neurite development; positive regulation of synaptic transmission; protein homooligomerization

Disease: Epilepsy, Familial Temporal Lobe, 1