MAK blocking peptide

Phospho-MAK (Thr157) Blocking Peptide

Cat. Num. AAA9618337

• Gene Names: MAK; RP62; dJ417M14.2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: MAK; Phospho-MAK (Thr157) Blocking Peptide; intestinal cell kinase; dJ417M14.2; fj04c02; Ick; Mak; MAK_HUMAN; Male germ cell associated kinase; Male germ cell-associated kinase; male germ cell-associated kinase retinal-enriched isoform; OTTHUMP00000016025; Rck; RP62; Serine/threonine protein kinase MAK; Serine/threonine-protein kinase MAK; testicular secretory protein Li 28; wu:fj04c02; zgc:56603; MAK blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for MAK blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MAK blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 334724436
• NCBI GeneID: 4117
• NCBI Accession #: NP_001229314.1
• NCBI GenBank Nucleotide #: NP_001229314.1
• UniProt Accession #: P20794; Q547D0; Q9NUH7; F1T0K6; G1FL29
• Molecular Weight: 70,581 Da
• NCBI Official Full Name: serine/threonine-protein kinase MAK isoform 2
• NCBI Official Synonym Full Names: male germ cell-associated kinase
• NCBI Official Symbol: MAK
• NCBI Official Synonym Symbols: RP62; dJ417M14.2
• NCBI Protein Information: serine/threonine-protein kinase MAK; serine/threonine protein kinase MAK; male germ cell-associated kinase retinal-enriched isoform
• UniProt Protein Name: Serine/threonine-protein kinase MAK
• Protein Family: Makatoxin
• UniProt Gene Name: MAK
• UniProt Entry Name: MAK_HUMAN

NCBI Description

The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

MAK: a protein kinase of the RCK family of kinases. It is expressed at high levels in the testis (primarily in germ cells), and in developing sensory epithelia. Localizes to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.22; Nuclear receptor co-regulator; Protein kinase, CMGC; CMGC group; RCK family

Chromosomal Location of Human Ortholog: 6p24

Cellular Component: centrosome; photoreceptor outer segment; photoreceptor inner segment; cytoplasm; midbody; nucleus

Molecular Function: protein binding; cyclin-dependent protein kinase activity; transcription coactivator activity; ATP binding; protein kinase activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; regulation of cell cycle; multicellular organismal development; protein amino acid autophosphorylation; photoreceptor cell maintenance; spermatogenesis; cell differentiation; protein amino acid phosphorylation

Disease: Retinitis Pigmentosa 62