NMDAR2B blocking peptide

Phospho-NMDAR2B (Tyr1070) Blocking Peptide

Cat. Num. AAA9616545

• Gene Names: GRIN2B; MRD6; NR2B; hNR3; GluN2B; NMDAR2B
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: NMDAR2B; Phospho-NMDAR2B (Tyr1070) Blocking Peptide; AW490526; EIEE27; Glutamate [NMDA] receptor subunit epsilon 2; Glutamate [NMDA] receptor subunit epsilon-2; Glutamate Receptor Ionotropic N Methyl D Aspartate 2B; Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B; Glutamate receptor ionotropic NMDA2B; Glutamate receptor subunit epsilon 2; Glutamate receptor; ionotropic; NMDA2B (epsilon 2); GRIN 2B; GRIN2B; hNR 3; hNR3; MGC142178; MGC142180; MRD6; N methyl D asparate receptor channel subunit epsilon 2; N methyl D aspartate receptor subtype 2B; N methyl D aspartate receptor subunit 2B; N methyl D aspartate receptor subunit 3; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NMDA NR2B; NMDA R2B; NMDE2; NMDE2_HUMAN; NME2; NR2B; NR3; NMDAR2B blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for NMDAR2B blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NMDAR2B blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 167003331
• NCBI GeneID: 2904
• NCBI Accession #: NP_000825.2
• NCBI GenBank Nucleotide #: NM_000834.3
• UniProt Accession #: Q13224; Q12919; Q13220; Q13225; Q14CU4; Q9UM56
• Molecular Weight: 166,367 Da
• NCBI Official Full Name: glutamate receptor ionotropic, NMDA 2B
• NCBI Official Synonym Full Names: glutamate receptor, ionotropic, N-methyl D-aspartate 2B
• NCBI Official Symbol: GRIN2B
• NCBI Official Synonym Symbols: MRD6; NR2B; hNR3; GluN2B; NMDAR2B
• NCBI Protein Information: glutamate receptor ionotropic, NMDA 2B; NR3; glutamate receptor subunit epsilon-2; N-methyl-D-aspartate receptor subunit 3; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2
• UniProt Protein Name: Glutamate receptor ionotropic, NMDA 2B
• UniProt Gene Name: GRIN2B
• UniProt Synonym Gene Names: NMDAR2B; GluN2B; NMDAR2B; NR2B; NR3; hNR3
• UniProt Entry Name: NMDE2_HUMAN

NCBI Description

N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008]

Uniprot Description

NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.

Protein type: Channel, calcium; Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12p12

Cellular Component: postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; integral to plasma membrane; dendrite; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; zinc ion binding; glycine binding; calcium channel activity; N-methyl-D-aspartate selective glutamate receptor activity

Biological Process: axon guidance; synaptic transmission, glutamatergic; behavioral fear response; startle response; in utero embryonic development; glutamate signaling pathway; regulation of synaptic plasticity; learning; memory; detection of mechanical stimulus involved in sensory perception of pain; synaptic transmission; behavioral response to pain; response to ethanol; sensory organ development; learning and/or memory; suckling behavior; transport; ionotropic glutamate receptor signaling pathway; ephrin receptor signaling pathway; regulation of excitatory postsynaptic membrane potential

Disease: Epileptic Encephalopathy, Early Infantile, 27; Mental Retardation, Autosomal Dominant 6