Western Blot (WB)
(Western blot analysis of EPHA8 (Phospho-Tyr616) using UV treated A549 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Rabbit EPHA8 Polyclonal Antibody | anti-EPHA8 antibody
Phospho-EPHA8 (Tyr616) Antibody
Gene Names
EPHA8; EEK; EK3; HEK3
Reactivity
Human, MousePredicted Reactivity: Pig (91%), Bovine (82%), Horse (100%), Sheep (82%), Rabbit (89%), Dog (82%)
Applications
Western Blot, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
EPHA8; Polyclonal Antibody; Phospho-EPHA8 (Tyr616) Antibody; EEK; EK3; EpHand ELK related kinase; EpHlike kinase 3; EpHreceptor A8; EPH- and ELK-related kinase; EPH-like kinase 3; EphA8; EPHA8_HUMAN; Ephrin Type A Receptor 8 Precursor; Ephrin type-A receptor 8; HEK3; KIAA1459; Tyrosine-protein kinase receptor EEK; Tyrosylprotein Kinase; anti-EPHA8 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted Reactivity: Pig (91%), Bovine (82%), Horse (100%), Sheep (82%), Rabbit (89%), Dog (82%)
Predicted Reactivity: Pig (91%), Bovine (82%), Horse (100%), Sheep (82%), Rabbit (89%), Dog (82%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-EPHA8 (Tyr616) Antibody detects endogenous levels of EPHA8 only when phosphorylated at Tyr616.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-EPHA8 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:1000-3000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human EPHA8 around the phosphorylation site of Tyr616.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated. Phosphorylation is stimulated upon binding of its ligands including EFNA2, EFNA3 and EFNA5. Autophosphorylation on Tyr-616 is critical for association with FYN. Autophosphorylation on Tyr-839 modulates tyrosine kinase activity (By similarity).Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation. ANKS1A prevents ubiquitination and degradation (By similarity).
Subunit Structure
Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. May also form heterodimers with other ephrin receptors (By similarity). Interacts with FYN; possible downstream effector of EPHA8 in regulation of cell adhesion. Interacts with PIK3CG; regulates integrin-mediated cell adhesion to substrate. Interacts with TIAM1; regulates clathrin-mediated endocytosis of EPHA8. Interacts with ANKS1A and ANKS1B; EPHA8 kinase activity-independent but stimulated by EPHA8 ubiquitination.
Similarity
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Subcellular Location
Cell membrane>Single-pass type I membrane protein. Cell projection. Early endosome membrane.
Note: Undergoes clathrin-mediated endocytosis upon EFNA5-binding and is targeted to early endosomes.
Note: Undergoes clathrin-mediated endocytosis upon EFNA5-binding and is targeted to early endosomes.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of EPHA8 (Phospho-Tyr616) using UV treated A549 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Western Blot (WB)
(Western blot analysis of EPHA8 (Phospho-Tyr616) using UV treated A549 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Related Product Information for anti-EPHA8 antibody
Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. The GPI-anchored ephrin-A EFNA2, EFNA3, and EFNA5 are able to activate EPHA8 through phosphorylation. With EFNA5 may regulate integrin-mediated cell adhesion and migration on fibronectin substrate but also neurite outgrowth. During development of the nervous system plays also a role in axon guidance. Downstream effectors of the EPHA8 signaling pathway include FYN which promotes cell adhesion upon activation by EPHA8 and the MAP kinases in the stimulation of neurite outgrowth (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
111,003 Da
NCBI Official Full Name
ephrin type-A receptor 8 isoform 2
NCBI Official Synonym Full Names
EPH receptor A8
NCBI Official Symbol
EPHA8
NCBI Official Synonym Symbols
EEK; EK3; HEK3
NCBI Protein Information
ephrin type-A receptor 8; EPH-like kinase 3; tyrosylprotein kinase; protein-tyrosine kinase; hydroxyaryl-protein kinase; EPH- and ELK-related kinase; EPH- and ELK-related tyrosine kinase; tyrosine-protein kinase receptor EEK
UniProt Protein Name
Ephrin type-A receptor 8
Protein Family
UniProt Gene Name
EPHA8
UniProt Synonym Gene Names
EEK; HEK3; KIAA1459; EK3; hEK3
UniProt Entry Name
EPHA8_HUMAN
Similar Products
Product Notes
The EPHA8 epha8 (Catalog #AAA9614145) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-EPHA8 (Tyr616) Antibody reacts with Human, Mouse Predicted Reactivity: Pig (91%), Bovine (82%), Horse (100%), Sheep (82%), Rabbit (89%), Dog (82%) and may cross-react with other species as described in the data sheet. AAA Biotech's EPHA8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:1000-3000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the EPHA8 epha8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EPHA8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
