Rabbit anti-Human Anillin Polyclonal Antibody | anti-ANLN antibody
Anillin Antibody
Gene Names
ANLN; scra; Scraps
Reactivity
Human
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
Anillin; Polyclonal Antibody; Anillin Antibody; Actin binding protein scraps homolog Drosophila; Actin binding protein anillin; Actin-binding protein anillin; Anillin actin binding protein; anln; ANLN_HUMAN; DKFZp779A055; FSGS8; Scra; Scraps; Scraps homolog; anti-ANLN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Anillin Antibody detects endogenous levels of total Anillin.
Tissue Specificity: Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus. Expression is significantly increased in renal biopsy specimens from idiopathic FSGS. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
Tissue Specificity: Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. In the kidney, it is widely expressed in tubules, but sparsely expressed in the glomerulus. Expression is significantly increased in renal biopsy specimens from idiopathic FSGS. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ANLN antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human Anillin.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated during mitosis.Ubiquitinated, and this requires FZR1/CDH1.
Subunit Structure
Interacts with F-actin. Interacts with CD2AP. May interact with RHOA. Interacts with FZR1/CDH1 during mitotic exit.
Subcellular Location
Nucleus. Cytoplasm>Cytoskeleton. Cytoplasm>Cell cortex. Cell projection>Bleb.
Note: Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
Note: Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Related Product Information for anti-ANLN antibody
Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. Plays a role in bleb assembly during metaphase and anaphase of mitosis. May play a significant role in podocyte cell migration.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
124,199 Da
NCBI Official Full Name
actin-binding protein anillin isoform 3
NCBI Official Synonym Full Names
anillin, actin binding protein
NCBI Official Symbol
ANLN
NCBI Official Synonym Symbols
scra; Scraps
NCBI Protein Information
actin-binding protein anillin
UniProt Protein Name
Actin-binding protein anillin
Protein Family
UniProt Gene Name
ANLN
UniProt Entry Name
ANLN_HUMAN
NCBI Description
This gene encodes an actin-binding protein that plays a role in cell growth and migration, and in cytokinesis. The encoded protein is thought to regulate actin cytoskeletal dynamics in podocytes, components of the glomerulus. Mutations in this gene are associated with focal segmental glomerulosclerosis 8. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
Uniprot Description
anillin: an actin binding protein that interacts with cleavage furrow proteins such as septins and may play a role in cytokinesis.
Protein type: Motility/polarity/chemotaxis; Actin-binding
Chromosomal Location of Human Ortholog: 7p15-p14
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; contractile ring; actin cytoskeleton
Molecular Function: actin binding
Biological Process: mitosis; cytokinesis after mitosis; septin ring assembly; regulation of exit from mitosis; hemopoietic progenitor cell differentiation
Disease: Focal Segmental Glomerulosclerosis 8
Research Articles on ANLN
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Product Notes
The ANLN anln (Catalog #AAA9613555) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anillin Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Anillin can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the ANLN anln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Anillin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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