Rabbit KIBRA Polyclonal Antibody | anti-KIBRA antibody

KIBRA Antibody

Cat. Num. AAA9613325

• Gene Names: WWC1; KIBRA; HBEBP3; HBEBP36; MEMRYQTL; PPP1R168
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (91%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Synonyms: KIBRA; Polyclonal Antibody; KIBRA Antibody; FLJ10865; FLJ23369; HBeAg-binding protein 3; HBEBP3; HBEBP36; KIAA0869; Kidney and brain protein; Protein KIBRA; Protein WWC1; WW and C2 domain containing 1; WW C2 and coiled-coil domain containing 1; WW domain-containing protein 1; WWC1; anti-KIBRA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (91%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: KIBRA Antibody detects endogenous levels of total KIBRA.; Tissue Specificity: Expressed in mammary epithelial cells and breast cancer cell lines. Found in the luminal epithelium surrounding the ducts in the normal breast. In the brain, expressed in somatodendritic compartment of neurons in the cortex and hippocampus and in the cerebellum it is found in the Purkinje cells and some granule cells (at protein level). Detected in brain, heart, colon and kidney. In the kidney, expressed in glomerular podocytes, in some tubules and in the collecting duct.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-KIBRA antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human KIBRA, corresponding to a region within C-terminal amino acids.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylation at Ser-542 and Ser-931 by CDK1 in response to spindle damage stress regulates mitotic exit, these two sites are dephosphorylated by CDC14B.
• Subunit Structure: Homodimer. Interacts with DDN. Interacts with DYNLL1 and histone H3. The interaction with DYNLL1 is mandatory for the recruitment and transactivation functions of ESR1 or DYNLL1 to the target chromatin and the interaction with histone H3 ensures proper regulatory interaction of WWC1-DYNLL1-ESR1 complexes with target chromatin. Interacts (via WW domains) with DDR1 (via PPxY motif) in a collagen-regulated manner. Interacts with PRKCZ (via the protein kinase domain). Forms a tripartite complex with DDR1 and PRKCZ, but predominantly in the absence of collagen. Interacts (via the ADDV motif) with PATJ (via PDZ domain 8). Interacts (via WW domains) with SYNPO (via PPxY motifs). Interacts with NF2 and SNX4.
• Similarity: The C2-domain mediates homodimerization. It is a calcium-sensitive lipid-binding domain with preference for PI (3)P.Belongs to the WWC family. KIBRA subfamily.
• Subcellular Location: Cytoplasm. Cytoplasm>Perinuclear region. Nucleus. Cell projection>Ruffle membrane.; Note: Colocalizes with PRKCZ in the perinuclear region.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of KIBRA in lysates of 293, using KIBRA Antibody(AF4617).)

Related Product Information for anti-KIBRA antibody

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. Modulates directional migration of podocytes. Acts as a substrate for PRKCZ. Plays a role in cognition and memory performance.

NCBI and Uniprot Product Information

• NCBI GI #: 242247251
• NCBI GeneID: 23286
• NCBI Accession #: NP_001155133.1
• NCBI GenBank Nucleotide #: NM_001161661.1
• UniProt Accession #: Q8IX03; O94946; Q6MZX4; Q6Y2F8; Q7Z4G8; Q8WVM4; Q9BT29; B4DK05
• Molecular Weight: 125,905 Da
• NCBI Official Full Name: protein KIBRA isoform 1
• NCBI Official Synonym Full Names: WW and C2 domain containing 1
• NCBI Official Symbol: WWC1
• NCBI Official Synonym Symbols: KIBRA; HBEBP3; HBEBP36; MEMRYQTL; PPP1R168
• NCBI Protein Information: protein KIBRA; HBeAg-binding protein 3; WW, C2 and coiled-coil domain containing 1; kidney and brain protein; protein WWC1; protein phosphatase 1, regulatory subunit 168
• UniProt Protein Name: Protein KIBRA
• Protein Family: Protein
• UniProt Gene Name: WWC1
• UniProt Synonym Gene Names: KIAA0869; KIBRA
• UniProt Entry Name: KIBRA_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic phosphoprotein that interacts with PRKC-zeta and dynein light chain-1. Alleles of this gene have been found that enhance memory in some individuals. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

KIBRA: a postsynaptic scaffolding protein associated with human memory performance. Interacts via its WW domains with the postsynaptic protein DDN, which plays a role in synaptic plasticity. Expressed in the hypothalamus and cerebellum, and in the hippocampus and cortex, both regions of the brain that are related to memory. Co-localizes with PKCZ in the mouse hippocampus. Binds to synaptopodin, modulating podocyte directional migration. Forms a complex with dynein light chain 1 that is recruited to ER-responsive promoters, and that is required for ER transactivation in breast cancer cells. Binds the minus end-directed microtubule motor dynein. Its amino terminus binds the eight PDZ domain of the polarity protein INADL . Two human isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: protein complex; perinuclear region of cytoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding, bridging; protein binding; transcription coactivator activity; protein complex scaffold; kinase binding

Biological Process: cell migration; regulation of intracellular transport; positive regulation of MAPKKK cascade; transcription, DNA-dependent; establishment of cell polarity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of organ growth

Disease: Memory Quantitative Trait Locus