Human Protein KIBRA ELISA Kit | WWC1 elisa kit

Human Protein KIBRA ELISA Kit

Cat. Num. AAA760854

• Gene Names: WWC1; KIBRA; HBEBP3; HBEBP36; MEMRYQTL; PPP1R168
• Reactivity: Human
• Synonyms: Protein KIBRA; Human Protein KIBRA ELISA Kit; WWC1/KIAA0869/KIBRA/WW domain-containing protein 1/HBeAg-binding protein 3/Kidney and brain protein; WWC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WWC1. No significant cross-reactivity or interference between WWC1 and analogues was observed.
• Sequence Length: 1118

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78-5000pg/ml
• Sensitivity: <46.9pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WWC1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WWC1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Standard Curve/Testing Data

Related Product Information for WWC1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- WWC1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- WWC1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the WWC1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of WWC1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 242247257
• NCBI GeneID: 23286
• NCBI Accession #: NP_001155134.1
• NCBI GenBank Nucleotide #: NM_001161662.1
• Molecular Weight: 125,905 Da
• NCBI Official Full Name: protein KIBRA isoform 2
• NCBI Official Synonym Full Names: WW and C2 domain containing 1
• NCBI Official Symbol: WWC1
• NCBI Official Synonym Symbols: KIBRA; HBEBP3; HBEBP36; MEMRYQTL; PPP1R168
• NCBI Protein Information: protein KIBRA
• UniProt Protein Name: Protein KIBRA
• Protein Family: Protein
• UniProt Gene Name: WWC1
• UniProt Synonym Gene Names: KIAA0869; KIBRA
• UniProt Entry Name: KIBRA_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic phosphoprotein that interacts with PRKC-zeta and dynein light chain-1. Alleles of this gene have been found that enhance memory in some individuals. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

KIBRA: a postsynaptic scaffolding protein associated with human memory performance. Interacts via its WW domains with the postsynaptic protein DDN, which plays a role in synaptic plasticity. Expressed in the hypothalamus and cerebellum, and in the hippocampus and cortex, both regions of the brain that are related to memory. Co-localizes with PKCZ in the mouse hippocampus. Binds to synaptopodin, modulating podocyte directional migration. Forms a complex with dynein light chain 1 that is recruited to ER-responsive promoters, and that is required for ER transactivation in breast cancer cells. Binds the minus end-directed microtubule motor dynein. Its amino terminus binds the eight PDZ domain of the polarity protein INADL . Two human isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cytoplasm; cytosol; nucleus; perinuclear region of cytoplasm; protein complex

Molecular Function: kinase binding; protein binding; protein binding, bridging; protein complex scaffold; transcription coactivator activity

Biological Process: cell migration; establishment of cell polarity; negative regulation of organ growth; negative regulation of transcription from RNA polymerase II promoter; positive regulation of MAPKKK cascade; regulation of intracellular transport; transcription, DNA-dependent

Disease: Memory Quantitative Trait Locus