Western Blot (WB)
(Western blot analysis of extracts from Mouse muscle, using RYR3 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit RYR3 Polyclonal Antibody | anti-RYR3 antibody
RYR3 Antibody
Gene Names
RYR3; RYR-3
Reactivity
Human, MousePredicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%)
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
RYR3; Polyclonal Antibody; RYR3 Antibody; Brain ryanodine receptor calcium release channel; Brain ryanodine receptor-calcium release channel; Brain type ryanodine receptor; Brain-type ryanodine receptor; HBRR; Ryanodine receptor 3; RYR 3; RYR-3; RyR3; RYR3_HUMAN; anti-RYR3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
RYR3 Antibody detects endogenous levels of total RYR3.
Tissue Specificity: Brain, skeletal muscle, placenta and possibly liver and kidney. In brain, highest levels are found in the cerebellum, hippocampus, caudate nucleus and amygdala, with lower levels in the corpus callosum, substantia nigra and thalamus.
Tissue Specificity: Brain, skeletal muscle, placenta and possibly liver and kidney. In brain, highest levels are found in the cerebellum, hippocampus, caudate nucleus and amygdala, with lower levels in the corpus callosum, substantia nigra and thalamus.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-RYR3 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human RYR3, corresponding to a region within N-terminal amino acids.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Homotetramer. Heterotetramer with RYR2. Interacts with CALM (By similarity). Interacts with FKBP1A. Interacts with SELENON (By similarity).
Similarity
The calcium release channel activity resides in the C-terminal region while the remaining part of the protein resides in the cytoplasm.Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR3 subfamily.
Subcellular Location
Sarcoplasmic reticulum membrane>Multi-pass membrane protein. Membrane>Multi-pass membrane protein. Microsome membrane>Multi-pass membrane protein. Sarcoplasmic reticulum.
Note: The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
Note: The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Mouse muscle, using RYR3 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse muscle, using RYR3 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-RYR3 antibody
Calcium channel that mediates the release of Ca (2+) from the sarcoplasmic reticulum into the cytoplasm in muscle and thereby plays a role in triggering muscle contraction. May regulate Ca (2+) release by other calcium channels. Calcium channel that mediates Ca (2+)-induced Ca (2+) release from the endoplasmic reticulum in non-muscle cells. Contributes to cellular calcium ion homeostasis (By similarity). Plays a role in cellular calcium signaling.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
551,484 Da
NCBI Official Full Name
ryanodine receptor 3
NCBI Official Synonym Full Names
ryanodine receptor 3
NCBI Official Symbol
RYR3
NCBI Official Synonym Symbols
RYR-3
NCBI Protein Information
ryanodine receptor 3
UniProt Protein Name
Ryanodine receptor 3
Protein Family
UniProt Gene Name
RYR3
UniProt Synonym Gene Names
HBRR; RYR-3; RyR3
NCBI Description
The protein encoded by this gene is a ryanodine receptor, which functions to release calcium from intracellular storage for use in many cellular processes. For example, the encoded protein is involved in skeletal muscle contraction by releasing calcium from the sarcoplasmic reticulum followed by depolarization of T-tubules. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
Calcium channel that mediates the release of Ca2+ from the sarcoplasmic reticulum into the cytoplasm in muscle and thereby plays a role in triggering muscle contraction. May regulate Ca2+ release by other calcium channels. Calcium channel that mediates Ca2+-induced Ca2+ release from the endoplasmic reticulum in non-muscle cells. Contributes to cellular calcium ion homeostasis (). Plays a role in cellular calcium signaling.
Research Articles on RYR3
Similar Products
Product Notes
The RYR3 ryr3 (Catalog #AAA9613319) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RYR3 Antibody reacts with Human, Mouse Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's RYR3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the RYR3 ryr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RYR3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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