Rabbit PAR4 Polyclonal Antibody | anti-PAR4 antibody

Phospho-PAR4 (Thr163) Antibody

Cat. Num. AAA9613285

• Gene Names: PAWR; PAR4; Par-4
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: PAR4; Polyclonal Antibody; Phospho-PAR4 (Thr163) Antibody; 2310001G03Rik; PAR 4; PAR-4; Pawr; PAWR_HUMAN; PRKC Apoptosis WT1 Regulator; PRKC apoptosis WT1 regulator protein; Prostate apoptosis response 4 protein; Prostate apoptosis response protein 4; prostate apoptosis response protein PAR-4; Transcriptional repressor Par-4-like protein PAWR; Transcriptional repressor PAR4; WT1 Interacting Protein; anti-PAR4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-PAR4 (Thr163) Antibody detects endogenous levels of PAR4 only when phosphorylated at Thr163.; Tissue Specificity: Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-PAR4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF/ICC: 1:100-1:500; IHC: 1:50-1:200; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human PAR4 around the phosphorylation site of Thr163.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Preferentially phosphorylated at the Thr-163 by PKC in cancer cells.
• Subunit Structure: Homooligomer. Interacts (via the C-terminal region) with WT1. Interacts with THAP1. Interacts with AATF. Interacts with BACE1. Interacts with SPSB1 (via B30.2/SPRY domain); this interaction is direct and occurs in association with the Elongin BC complex. Interacts with SPSB2 (via B30.2/SPRY domain); this interaction occurs in association with the Elongin BC complex. Interacts with SPSB4 (via B30.2/SPRY domain); this interaction occurs in association with the Elongin BC complex. Component of a ternary complex composed of SQSTM1 and PRKCZ. Interacts with actin (By similarity).
• Similarity: The leucine-zipper domain is not essential for apoptosis, but is required for sensitization of cells to exogenous apoptotic insults and for interaction with its partners.The SAC domain is a death-inducing domain selective for apoptosis induction in cancer cells. This domain is essential for nuclear entry, Fas activation, inhibition of NF-kappa-B activity and induction of apoptosis in cancer cells (By similarity).The B30.2/SPRY domain-binding motif mediates recognition by proteins containing a B30.2/SPRY domain.
• Subcellular Location: Cytoplasm. Nucleus.; Note: Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from PC12(UV treatment), using Phospho-PAR4 (Thr163) Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa  , using Phospho-PAR-4 (Thr163) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Immunohistochemistry (IHC)

(At 1/100 staining Rat muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Testing Data

(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Related Product Information for anti-PAR4 antibody

Pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.

NCBI and Uniprot Product Information

• NCBI GI #: 55769533
• NCBI GeneID: 5074
• NCBI Accession #: NP_002574.2
• NCBI GenBank Nucleotide #: NM_002583.2
• UniProt Accession #: Q96IZ0; O75796; Q6FHY9; Q8N700
• Molecular Weight: 36,568 Da
• NCBI Official Full Name: PRKC apoptosis WT1 regulator protein
• NCBI Official Synonym Full Names: PRKC, apoptosis, WT1, regulator
• NCBI Official Symbol: PAWR
• NCBI Official Synonym Symbols: PAR4; Par-4
• NCBI Protein Information: PRKC apoptosis WT1 regulator protein; WT1-interacting protein; prostate apoptosis response-4; transcriptional repressor PAR4; prostate apoptosis response 4 protein; prostate apoptosis response protein 4; prostate apoptosis response protein PAR-4
• UniProt Protein Name: PRKC apoptosis WT1 regulator protein
• UniProt Gene Name: PAWR
• UniProt Synonym Gene Names: PAR4; Par-4
• UniProt Entry Name: PAWR_HUMAN

NCBI Description

The tumor suppressor WT1 represses and activates transcription. The protein encoded by this gene is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells. [provided by RefSeq, Jul 2008]

Uniprot Description

PAR-4: a proapoptotic protein that drives trafficking and activation of Fas and Fasl to induce prostate cancer cell apoptosis and tumor regression. Capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappaB transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 12q21

Cellular Component: cytoplasm; plasma membrane; actin filament; nucleus; actin cytoskeleton

Molecular Function: leucine zipper domain binding; protein binding; enzyme binding; actin binding; transcription corepressor activity

Biological Process: negative regulation of cell proliferation; actin filament bundle formation; negative regulation of T cell proliferation; interleukin-2 biosynthetic process; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; negative regulation of T cell receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of B cell proliferation; positive regulation of amyloid precursor protein biosynthetic process