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Western Blot (WB) (Western blot analysis of extracts from Rat heart, using Phospho-XIRP1 (Ser295) Antibody. The lane on the left was treated with blocking peptide.Observed bands: 110kDa)

Rabbit XIRP1 Polyclonal Antibody | anti-XIRP1 antibody

Phospho-XIRP1 (Ser295) Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
XIRP1; Polyclonal Antibody; Phospho-XIRP1 (Ser295) Antibody; anti-XIRP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-XIRP1 (Ser295) Antibody detects endogenous levels of XIRP1 only when phosphorylated at Ser295.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-XIRP1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
Application Notes
IHC: 1:50-1:200
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human XIRP1 around the phosphorylation site of Ser295.
Conjugation
Unconjugated
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Rat heart, using Phospho-XIRP1 (Ser295) Antibody. The lane on the left was treated with blocking peptide.Observed bands: 110kDa)

Western Blot (WB) (Western blot analysis of extracts from Rat heart, using Phospho-XIRP1 (Ser295) Antibody. The lane on the left was treated with blocking peptide.Observed bands: 110kDa)

Immunohistochemistry (IHC)

(Staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC) (Staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)

(At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

NCBI and Uniprot Product Information

NCBI GI #
UniProt Accession #
Molecular Weight
123,431 Da
NCBI Official Full Name
Xin actin-binding repeat-containing protein 1
UniProt Protein Name
Xin actin-binding repeat-containing protein 1
UniProt Gene Name
Xirp1
UniProt Synonym Gene Names
Cmya1; Xin

Uniprot Description

CMYA1: Protects actin filaments from depolymerization. Belongs to the Xin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding; Cell adhesion; Cell development/differentiation

Chromosomal Location of Human Ortholog: 9|9 F4

Cellular Component: cell junction; cell-cell adherens junction; fascia adherens; filamentous actin; focal adhesion; intercalated disc; stress fiber

Molecular Function: actin binding; filamin binding; RNA binding

Biological Process: actin cytoskeleton organization; cardiac muscle cell development; heart development; heart morphogenesis; negative regulation of cell proliferation; negative regulation of protein binding; Notch signaling pathway; regulation of membrane potential; sarcomere organization

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Product Notes

The XIRP1 xirp1 (Catalog #AAA9612830) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-XIRP1 (Ser295) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's XIRP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). IHC: 1:50-1:200 WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the XIRP1 xirp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XIRP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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