Rabbit Intersectin 1 Polyclonal Antibody | anti-ITSN1 antibody

Intersectin 1 Antibody

Cat. Num. AAA9612486

• Gene Names: ITSN1; ITSN; SH3D1A; SH3P17
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: Peptide affinity purification
• Synonyms: Intersectin 1; Polyclonal Antibody; Intersectin 1 Antibody; Human intersectin SH3 domain containing protein SH3P17; Intersectin 1 SH3 domain protein; intersectin 1 short form variant 3; intersectin 1 short form variant; 11; Intersectin short form; intersectin short variant 12; Intersectin-1; Intersectin1; ITSN 1; ITSN; itsn1; ITSN1_HUMAN; MGC134948; MGC134949; OTTHUMP00000067855; OTTHUMP00000067856; OTTHUMP00000067857; OTTHUMP00000194810; OTTHUMP00000194811; OTTHUMP00000194812; OTTHUMP00000206688; SH3 domain containing protein 1A; SH3 domain protein 1A; SH3 domain-containing protein 1A; SH3D1A; SH3P17; Src homology 3 domain containing protein; anti-ITSN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Intersectin 1 Antibody detects endogenous levels of total Intersectin 1.
• Purity/Purification: Peptide affinity purification
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1220

• Applicable Applications for anti-ITSN1 antibody: ELISA (EIA)
• Application Notes: IF: 1:100-1:500; ICC: 1:100-1:500; WB: 1:500-1:2000; ELISA(peptide): 1:20,000-1:40,000B; *The optimal dilutions should be determined by the end user. ; ; IMPORTANT: For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Immunogen: A synthesized peptide derived from human Intersectin 1, corresponding to a region within N-terminal amino acids.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Human Fetal brain, using Intersectin 1 Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB)

(Western blot analysis of extracts from Mouse brain, using Intersectin 1 Antibody. The lane on the left was treated with blocking peptide.)

Immunofluorescence (IF)/Immunocytochemistry (ICC)

(MBS9612486 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612486 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Related Product Information for anti-ITSN1 antibody

Acts as guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex (PubMed:11584276). Plays a role in the assembly and maturation of clathrin-coated vesicles (By similarity). Recruits FCHSD2 to clathrin-coated pits (PubMed:29887380). Involved in endocytosis of activated EGFR, and probably also other growth factor receptors (By similarity). Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2 (PubMed:22648170). Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines. Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones (By similarity). Inhibits ARHGAP31 activity toward RAC1.

NCBI and Uniprot Product Information

• NCBI GI #: 47717125
• NCBI GeneID: 6453
• NCBI Accession #: NP_001001132.1
• NCBI GenBank Nucleotide #: NP_001001132.1
• UniProt Accession #: Q15811
• Molecular Weight: 195kDa
• NCBI Official Full Name: intersectin-1 isoform ITSN-s
• NCBI Official Synonym Full Names: intersectin 1
• NCBI Official Symbol: ITSN1
• NCBI Official Synonym Symbols: ITSN; SH3D1A; SH3P17
• NCBI Protein Information: intersectin-1
• UniProt Protein Name: Intersectin-1
• Protein Family: Intersectin
• UniProt Gene Name: ITSN1
• UniProt Synonym Gene Names: ITSN; SH3D1A
• UniProt Entry Name: ITSN1_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far. [provided by RefSeq, Jul 2008]