Rabbit TIM 4 Polyclonal Antibody | anti-TIMD4 antibody

TIM 4 Antibody

Cat. Num. AAA9612395

• Gene Names: TIMD4; TIM4; SMUCKLER
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: TIM 4; Polyclonal Antibody; TIM 4 Antibody; SMUCKLER; Spleen; mucin-containing; knockout of lymphotoxin protein; T cell immunoglobulin and mucin domain containing protein 4; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain containing molecule; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin and mucin domains-containing protein 4; T-cell membrane protein 4; TIM-4; Tim4; TIMD 4; TIMD-4; Timd4; TIMD4_HUMAN; anti-TIMD4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TIM 4 Antibody detects endogenous levels of total TIM 4.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 378

• Applicable Applications for anti-TIMD4 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IF: 1:100-1:500; ICC: 1:100-1:500; IHC: 1:50-1:200; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human TIM 4, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Jurkat, using TIM 4 Antibody. The lane on the left was treated with blocking peptide.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612395 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612395 at 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612395 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612395 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612395 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612395 at 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612395 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612395 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612395 at 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Related Product Information for anti-TIMD4 antibody

Function: Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1 (By similarity).

Subcellular Location: Membrane>Single-pass type I membrane protein.

Similarity: Recognizes phosphatidyl serine via its immunoglobulin domain.Belongs to the immunoglobulin superfamily. TIM family.

NCBI and Uniprot Product Information

• NCBI GI #: 226529863
• NCBI GeneID: 91937
• NCBI Accession #: NP_612388
• NCBI GenBank Nucleotide #: NP_612388.2
• UniProt Accession #: Q96H15
• Molecular Weight: Observed Molecular Weight: (Observed)41kD.; Predicted Molecular Weight: (Calculated)42kDa.
• NCBI Official Full Name: T-cell immunoglobulin and mucin domain-containing protein 4 isoform 1
• NCBI Official Synonym Full Names: T cell immunoglobulin and mucin domain containing 4
• NCBI Official Symbol: TIMD4
• NCBI Official Synonym Symbols: TIM4; SMUCKLER
• NCBI Protein Information: T-cell immunoglobulin and mucin domain-containing protein 4
• UniProt Protein Name: T-cell immunoglobulin and mucin domain-containing protein 4
• Protein Family: T-cell immunoglobulin and mucin domain-containing protein
• UniProt Gene Name: TIMD4
• UniProt Synonym Gene Names: TIM4; TIMD-4; TIM-4
• UniProt Entry Name: TIMD4_HUMAN

Uniprot Description

TIM-4: Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1. Belongs to the immunoglobulin superfamily. TIM family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q33.3

Cellular Component: integral to membrane