Rabbit CD43 Polyclonal Antibody | anti-SPN antibody

CD43 Antibody

Cat. Num. AAA9612319

• Gene Names: SPN; LSN; CD43; GALGP; GPL115
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Horse(86%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: CD43; Polyclonal Antibody; CD43 Antibody; CD 43; CD43 antigen; Galactoglycoprotein; GALGP; GPL 115; GPL115; Human gene for sialophorin; Leucocyte sialoglycoprotein; LEUK_HUMAN; Leukocyte large sialoglycoprotein; Leukocyte sialoglycoprotein; Leukosialin; LSN; Ly-48; sialophorin (gpL115; leukosialin; CD43); Sialophorin; Spn; anti-SPN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Horse(86%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CD43 Antibody detects endogenous levels of total CD43.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 400

• Applicable Applications for anti-SPN antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human CD43, corresponding to a region within the internal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Human tonsil, using CD43 Antibody. The lane on the left was treated with blocking peptide.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612319 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Related Product Information for anti-SPN antibody

Function: Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity).Protects cells from apoptotic signals, promoting cell survival.

Post Translational Modifications: Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.Phosphorylation at Ser-355 is regulated by chemokines, requires its association with ERM proteins (EZR, RDX and MSN) and is essential for its function in the regulation of T-cell trafficking to lymph nodes.Has a high content of sialic acid and O-linked carbohydrate structures.Cleavage by CTSG releases its extracellular domain and triggers its intramembrane proteolysis by gamma-secretase releasing the CD43 cytoplasmic tail chain (CD43-ct) which translocates to the nucleus.Sumoylated.

Subcellular Location: Membrane>Single-pass type I membrane protein. Cell projection>Microvillus. Cell projection>Uropodium. Note: Localizes to the uropodium and microvilli via its interaction with ERM proteins (EZR, RDX and MSN).Nucleus. Nucleus>PML body.

Tissue Specificity: Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Subunit Structure: Interacts with HIPK2 via the cytoplasmic domain. Interacts with SIGLEC1, RDX, EZR and MSN.

NCBI and Uniprot Product Information

• NCBI GI #: 71979937
• NCBI GeneID: 6693
• NCBI Accession #: NP_001025459.1
• NCBI GenBank Nucleotide #: NP_001025459.1
• UniProt Accession #: P16150
• Molecular Weight: Observed Molecular Weight: (Observed)40kD.; Predicted Molecular Weight: (Calculated)40kDa.
• NCBI Official Full Name: leukosialin
• NCBI Official Synonym Full Names: sialophorin
• NCBI Official Symbol: SPN
• NCBI Official Synonym Symbols: LSN; CD43; GALGP; GPL115
• NCBI Protein Information: leukosialin
• UniProt Protein Name: Leukosialin
• Protein Family: Leukosialin
• UniProt Gene Name: SPN
• UniProt Synonym Gene Names: CD43; GALGP
• UniProt Entry Name: LEUK_HUMAN

NCBI Description

This gene encodes a highly sialylated glycoprotein that functions in antigen-specific activation of T cells, and is found on the surface of thymocytes, T lymphocytes, monocytes, granulocytes, and some B lymphocytes. It contains a mucin-like extracellular domain, a transmembrane region and a carboxy-terminal intracellular region. The extracellular domain has a high proportion of serine and threonine residues, allowing extensive O-glycosylation, and has one potential N-glycosylation site, while the carboxy-terminal region has potential phosphorylation sites that may mediate transduction of activation signals. Different glycoforms of this protein have been described. In stimulated immune cells, proteolytic cleavage of the extracellular domain occurs in some cell types, releasing a soluble extracellular fragment. Defects in expression of this gene are associated with Wiskott-Aldrich syndrome. [provided by RefSeq, Sep 2017]

Uniprot Description

sialophorin: One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter-receptor for SN/Siglec-1. During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. Interacts with HIPK2 via the cytoplasmic domain. Interacts with RDX. Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: extracellular space; cell surface; membrane; integral to plasma membrane; plasma membrane; basement membrane; uropod; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; regulation of defense response to virus; establishment and/or maintenance of cell polarity; negative regulation of cell adhesion; signal transduction; chemotaxis; negative thymic T cell selection; response to protozoan; negative regulation of T cell proliferation; cell surface receptor linked signal transduction; T cell costimulation; defense response to bacterium; cellular defense response; positive regulation of T cell proliferation; immune response; positive regulation of protein amino acid phosphorylation; negative regulation of type IV hypersensitivity; blood coagulation; leukocyte migration