Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using KLF15 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit KLF15 Polyclonal Antibody | anti-KLF15 antibody
KLF15 Antibody
Gene Names
KLF15; KKLF
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
KLF15; Polyclonal Antibody; KLF15 Antibody; Kidney enriched Kruppel like factor; Kidney-enriched krueppel-like factor; KKLF; KKLF protein; KLF 15; KLF15_HUMAN; Krueppel-like factor 15; Kruppel like factor 15; anti-KLF15 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
KLF15 Antibody detects endogenous levels of total KLF15.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
416
Applicable Applications for anti-KLF15 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human KLF15, corresponding to a region within N-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using KLF15 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using KLF15 Antibody. The lane on the left was treated with blocking peptide.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612106 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612106 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-KLF15 antibody
Function: Transcriptional regulator that binds to the GA element of the CLCNKA promoter. Binds to the KCNIP2 promoter and regulates KCNIP2 circadian expression in the heart (By similarity). Is a repressor of CCN2 expression, involved in the control of cardiac fibrosis. It is also involved in the control of cardiac hypertrophy acting through the inhibition of MEF2A and GATA4 (By similarity). Involved in podocyte differentiation (By similarity). Inhibits MYOCD activity. Is a negative regulator of TP53 acetylation. Inhibits NF-kappa-B activation through repression of EP300-dependent RELA acetylation.
Subcellular Location: Nucleus.
Tissue Specificity: Highly expressed in liver, skeletal muscle, and kidney. Expressed in cardiomyocytes. Expression is highly reduced in cardiac tissue of patients with non-ischemic cardiomyopathy and aortic aneurysm, and in glomerular disease. Not expressed in bone marrow or lymphoid tissues.
Subunit Structure: Interacts with MYOCD and EP300.
Similarity: The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.Belongs to the Sp1 C2H2-type zinc-finger protein family.
Subcellular Location: Nucleus.
Tissue Specificity: Highly expressed in liver, skeletal muscle, and kidney. Expressed in cardiomyocytes. Expression is highly reduced in cardiac tissue of patients with non-ischemic cardiomyopathy and aortic aneurysm, and in glomerular disease. Not expressed in bone marrow or lymphoid tissues.
Subunit Structure: Interacts with MYOCD and EP300.
Similarity: The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.Belongs to the Sp1 C2H2-type zinc-finger protein family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)43kD.
Predicted Molecular Weight: (Calculated)44kDa.
Predicted Molecular Weight: (Calculated)44kDa.
NCBI Official Full Name
Krueppel-like factor 15
NCBI Official Synonym Full Names
Kruppel like factor 15
NCBI Official Symbol
KLF15
NCBI Official Synonym Symbols
KKLF
NCBI Protein Information
Krueppel-like factor 15
UniProt Protein Name
Krueppel-like factor 15
Protein Family
UniProt Gene Name
KLF15
UniProt Synonym Gene Names
KKLF
UniProt Entry Name
KLF15_HUMAN
Uniprot Description
KLF15: Transcriptional activator. Binds to the GA element of the CLCNKA promoter. Belongs to the Sp1 C2H2-type zinc-finger protein family.
Protein type: Transcription factor; C2H2-type zinc finger protein; DNA-binding
Chromosomal Location of Human Ortholog: 3q21.3
Cellular Component: nucleus
Molecular Function: protein binding; metal ion binding
Biological Process: cardiac muscle hypertrophy; glial cell differentiation; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; glucose transport
Research Articles on KLF15
Similar Products
Product Notes
The KLF15 klf15 (Catalog #AAA9612106) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KLF15 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's KLF15 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the KLF15 klf15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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