Western Blot (WB)
(Western blot analysis of extracts from RPE, using Bestrophin Antibody. The lane on the left was treated with blocking peptide.)
Rabbit Bestrophin Polyclonal Antibody | anti-BEST1 antibody
Bestrophin Antibody
Gene Names
BEST1; ARB; BMD; BEST; RP50; VMD2; TU15B; Best1V1Delta2
Reactivity
Human, Mouse, Rat, BovinePredicted Reactivity: Horse(92%), Sheep(100%), Rabbit(92%), Dog(92%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Bestrophin; Polyclonal Antibody; Bestrophin Antibody; ARB; BEST 1; BEST; Best disease; Best macular dystrophy; BEST1; BEST1_HUMAN; Best1V1Delta2; Bestrophin 1; Bestrophin-1; Bestrophin1; BMD; mBest1; RP50; TU15B; Vitelliform macular dystrophy 2; Vitelliform macular dystrophy; Vitelliform macular dystrophy protein 2; VMD 2; VMD2; anti-BEST1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine
Predicted Reactivity: Horse(92%), Sheep(100%), Rabbit(92%), Dog(92%)
Predicted Reactivity: Horse(92%), Sheep(100%), Rabbit(92%), Dog(92%)
Clonality
Polyclonal
Isotype
IgG
Specificity
Bestrophin Antibody detects endogenous levels of total Bestrophin.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
604
Applicable Applications for anti-BEST1 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human Bestrophin, corresponding to a region within N-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from RPE, using Bestrophin Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from RPE, using Bestrophin Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-BEST1 antibody
Function: Forms calcium-sensitive chloride channels. Highly permeable to bicarbonate.
Post Translational Modifications: Phosphorylated by PP2A.
Subcellular Location: Cell membrane>Multi-pass membrane protein. Basolateral cell membrane.
Tissue Specificity: Predominantly expressed in the basolateral membrane of the retinal pigment epithelium.
Subunit Structure: Homooligomer (tetramer or pentamer) (PubMed:26200502). May interact with PPP2CB and PPP2R1B (By similarity).
Similarity: Belongs to the bestrophin family.
Post Translational Modifications: Phosphorylated by PP2A.
Subcellular Location: Cell membrane>Multi-pass membrane protein. Basolateral cell membrane.
Tissue Specificity: Predominantly expressed in the basolateral membrane of the retinal pigment epithelium.
Subunit Structure: Homooligomer (tetramer or pentamer) (PubMed:26200502). May interact with PPP2CB and PPP2R1B (By similarity).
Similarity: Belongs to the bestrophin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)67kD.
Predicted Molecular Weight: (Calculated)68kDa.
Predicted Molecular Weight: (Calculated)68kDa.
NCBI Official Full Name
bestrophin-1 isoform 2
NCBI Official Synonym Full Names
bestrophin 1
NCBI Official Symbol
BEST1
NCBI Official Synonym Symbols
ARB; BMD; BEST; RP50; VMD2; TU15B; Best1V1Delta2
NCBI Protein Information
bestrophin-1
UniProt Protein Name
Bestrophin-1
Protein Family
UniProt Gene Name
BEST1
UniProt Synonym Gene Names
VMD2
UniProt Entry Name
BEST1_HUMAN
NCBI Description
This gene encodes a member of the bestrophin gene family. This small gene family is characterized by proteins with a highly conserved N-terminus with four to six transmembrane domains. Bestrophins may form chloride ion channels or may regulate voltage-gated L-type calcium-ion channels. Bestrophins are generally believed to form calcium-activated chloride-ion channels in epithelial cells but they have also been shown to be highly permeable to bicarbonate ion transport in retinal tissue. Mutations in this gene are responsible for juvenile-onset vitelliform macular dystrophy (VMD2), also known as Best macular dystrophy, in addition to adult-onset vitelliform macular dystrophy (AVMD) and other retinopathies. Alternative splicing results in multiple variants encoding distinct isoforms.[provided by RefSeq, Nov 2008]
Research Articles on BEST1
Similar Products
Product Notes
The BEST1 best1 (Catalog #AAA9612057) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bestrophin Antibody reacts with Human, Mouse, Rat, Bovine Predicted Reactivity: Horse(92%), Sheep(100%), Rabbit(92%), Dog(92%) and may cross-react with other species as described in the data sheet. AAA Biotech's Bestrophin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the BEST1 best1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bestrophin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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