Western Blot (WB)
(Western blot analysis UBE4B using Jurkat whole cell lysates)
Rabbit anti-Human, Mouse UBE4B Polyclonal Antibody | anti-UBE4B antibody
UBE4B Antibody
Gene Names
UBE4B; E4; UFD2; HDNB1; UBOX3; UFD2A
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
UBE4B; Polyclonal Antibody; UBE4B Antibody; 4930551I19Rik; 4933406G05Rik; AU014668; D4Bwg0973e; E4; HDNB1; Homozygously deleted in neuroblastoma 1; KIAA0684; mKIAA0684; RP23 173D8.1; Ube4b; UBE4B_HUMAN; Ubiquitin conjugation factor E4 B; Ubiquitin fusion degradation protein 2; UBOX3; UFD2; UFD2a; Ufd2p; anti-UBE4B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
UBE4B antibody detects endogenous levels of total UBE4B
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1302
Applicable Applications for anti-UBE4B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human UBE4B
Subcellular Location
Cytoplasm.
Tissue Specificity
Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (86%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (92%), Dog (92%), Chicken (86%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis UBE4B using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis UBE4B using Jurkat whole cell lysates)
Related Product Information for anti-UBE4B antibody
Function: Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases. May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase.
Subunit Structure: Interacts with VCP.
Post-translational Modifications: Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
Similarity: The U-box domain is required for the ubiquitin protein ligase activity. Belongs to the ubiquitin conjugation factor E4 family.
Subunit Structure: Interacts with VCP.
Post-translational Modifications: Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
Similarity: The U-box domain is required for the ubiquitin protein ligase activity. Belongs to the ubiquitin conjugation factor E4 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 146 kDa
Predicted: 147 kDa
Predicted: 147 kDa
NCBI Official Full Name
ubiquitin conjugation factor E4 B isoform 1
NCBI Official Synonym Full Names
ubiquitination factor E4B
NCBI Official Symbol
UBE4B
NCBI Official Synonym Symbols
E4; UFD2; HDNB1; UBOX3; UFD2A
NCBI Protein Information
ubiquitin conjugation factor E4 B
UniProt Protein Name
Ubiquitin conjugation factor E4 B
Protein Family
UniProt Gene Name
UBE4B
UniProt Synonym Gene Names
HDNB1ImportedManual assertion inferred from database entriesiEMBL:BAB40446.1; KIAA0684ImportedManual assertion inferred from database entriesiEMBL:BAA31659.3; UFD2ImportedManual assertion inferred from database entriesiEMBL:AAD02233.1
NCBI Description
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (PubMed:17369820).
Research Articles on UBE4B
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Product Notes
The UBE4B ube4b (Catalog #AAA9610311) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE4B Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's UBE4B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the UBE4B ube4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE4B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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