Western Blot (WB)
(Western blot analysis USP31 using COLO205 whole cell lysates)
Rabbit USP31 Polyclonal Antibody | anti-USP31 antibody
USP31 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
USP31; Polyclonal Antibody; USP31 Antibody; Deubiquitinating enzyme 31; KIAA1203; Ubiquitin carboxyl terminal hydrolase 31; Ubiquitin carboxyl-terminal hydrolase 31; Ubiquitin specific peptidase 31; Ubiquitin specific processing protease 31; Ubiquitin specific protease 31; Ubiquitin specific proteinase 31; Ubiquitin thioesterase 31; Ubiquitin thiolesterase 31; Ubiquitin-specific-processing protease 31; UBP31_HUMAN; USP 31; anti-USP31 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
USP31 antibody detects endogenous levels of total USP31
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1352
Applicable Applications for anti-USP31 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human USP31
Tissue Specificity
Widely expressed.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (92%), Bovine (92%), Horse (92%), Sheep (92%), Dog (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis USP31 using COLO205 whole cell lysates)
Western Blot (WB)
(Western blot analysis USP31 using COLO205 whole cell lysates)
Immunohistochemistry (IHC)
(MBS9610303 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9610303 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-USP31 antibody
Function: May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).
Similarity: Belongs to the peptidase C19 family.
Similarity: Belongs to the peptidase C19 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 147 kDa
Predicted: 147 kDa
Predicted: 147 kDa
NCBI Official Full Name
ubiquitin carboxyl-terminal hydrolase 31
NCBI Official Synonym Full Names
ubiquitin specific peptidase 31
NCBI Official Symbol
USP31
NCBI Protein Information
ubiquitin carboxyl-terminal hydrolase 31
UniProt Protein Name
Ubiquitin carboxyl-terminal hydrolase 31
Protein Family
UniProt Gene Name
USP31
UniProt Synonym Gene Names
KIAA1203
Uniprot Description
May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins ().
Similar Products
Product Notes
The USP31 usp31 (Catalog #AAA9610303) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The USP31 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's USP31 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the USP31 usp31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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