Rabbit anti-Human, Mouse SH3PXD2A Polyclonal Antibody | anti-SH3PXD2A antibody

SH3PXD2A Antibody

Cat. Num. AAA9610218

• Gene Names: SH3PXD2A; FISH; TKS5; SH3MD1
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (ThermoFisher Scientific).
• Synonyms: SH3PXD2A; Polyclonal Antibody; SH3PXD2A Antibody; Adapter protein TKS5; Five SH3 domain-containing protein; SH3 and PX domain-containing protein 2A; SH3 multiple domains protein 1; Sh3md1; Sh3pxd2a; SPD2A_HUMAN; TKs5; Tyrosine kinase substrate with five SH3 domains; anti-SH3PXD2A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SH3PXD2A antibody detects endogenous levels of total SH3PXD2A
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (ThermoFisher Scientific).
• Form/Format: Liquid; Rabbit IgG in Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1105

• Applicable Applications for anti-SH3PXD2A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; EIA(peptide): 1:20000-1:40000; ; IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Immunogen: A synthesized peptide derived from human SH3PXD2A, corresponding to a region within N-terminal amino acids.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis SH3PXD2A using NIH-3T3 whole cell lysates)

Related Product Information for anti-SH3PXD2A antibody

Function: Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1-or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.
Subunit Structure: Interacts (via N-terminus) with CYBA (By similarity). Interacts with ADAM12, ADAM15 and ADAM19. Interacts with NOXO1. Interacts (via SH3 domains) with NOXA1. Interacts with FASLG.
Post-translational Modifications: Tyrosine phosphorylated by SRC. Phosphorylation plays a regulatory role in the protein localization. The intramolecular interaction of the PX domain with the third SH3 domain maintains the protein in the cytoplasm and phosphorylation disrupts this interaction, resulting in the redistribution of the protein from cytoplasm to the perimembrane region. Phosphorylated on serine upon DNA damage, probably by ATM or ATR.
Similarity: The PX domain is required for podosome localization because of its ability to bind phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3, 4-bisphosphate (PtdIns(3, 4)P2) and, to a lesser extent, phosphatidylinositol 4-phosphate (PtdIns4P), phosphatidylinositol 5-phosphate (PtdIns5P), and phosphatidylinositol 3, 5-bisphosphate (PtdIns(3, 5)P2). Binds to the third intramolecular SH3 domain (By similarity).The fifth SH3 domain mediates binding with ADAM12, ADAM15 and ADAM19. Belongs to the SH3PXD2 family.

NCBI and Uniprot Product Information

• NCBI GI #: 55749544
• NCBI GeneID: 9644
• NCBI Accession #: NP_055446.2
• NCBI GenBank Nucleotide #: NM_014631.2
• UniProt Accession #: Q5TCZ1; O43302; Q5TCZ2; Q5TDQ8; D3DR98
• Molecular Weight: 125 kDa
• NCBI Official Full Name: SH3 and PX domain-containing protein 2A isoform 1
• NCBI Official Synonym Full Names: SH3 and PX domains 2A
• NCBI Official Symbol: SH3PXD2A
• NCBI Official Synonym Symbols: FISH; TKS5; SH3MD1
• NCBI Protein Information: SH3 and PX domain-containing protein 2A
• UniProt Protein Name: SH3 and PX domain-containing protein 2A
• Protein Family: SH3 and PX domain-containing protein
• UniProt Gene Name: SH3PXD2A
• UniProt Synonym Gene Names: FISH; KIAA0418; SH3MD1; TKS5

Uniprot Description

Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.