Western Blot (WB)
(Western blot analysis of AMOT using Jurkat whole cell lysates)
Rabbit anti-Human, Mouse AMOT Polyclonal Antibody | anti-AMOT antibody
AMOT Antibody
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
AMOT; Polyclonal Antibody; AMOT Antibody; AMOT_HUMAN; Angiomotin; Angiomotin p130 isoform; KIAA1071; anti-AMOT antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
AMOT antibody detects endogenous levels of total AMOT
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1084
Applicable Applications for anti-AMOT antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human AMOT
Subcellular Location
Cell Junction > Tight Junction. Localized on the cell surface. May act as a transmembrane protein.
Tissue Specificity
Expressed in placenta and skeletal muscle. Found in the endothelial cells of capillaries as well as larger vessels of the placenta.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of AMOT using Jurkat whole cell lysates)
Western Blot (WB)
(Western blot analysis of AMOT using Jurkat whole cell lysates)
Related Product Information for anti-AMOT antibody
Function: Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
Subunit Structure: Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, PATJ and PARD3/PAR3. Interacts with MAGI1. Isoform 1 interacts with angiostatin.
Post-translational Modifications: Polyubiquitinated by NEDD4, NEDD4L and ITCH, leading to proteasomal degradation.
Similarity: The coiled coil domain interacts directly with the BAR domain of ARHGAP17.The angiostatin binding domain (871-1005) allows the binding to angiostatin. Belongs to the angiomotin family.
Subunit Structure: Component of a complex whose core is composed of ARHGAP17, AMOT, MPP5/PALS1, PATJ and PARD3/PAR3. Interacts with MAGI1. Isoform 1 interacts with angiostatin.
Post-translational Modifications: Polyubiquitinated by NEDD4, NEDD4L and ITCH, leading to proteasomal degradation.
Similarity: The coiled coil domain interacts directly with the BAR domain of ARHGAP17.The angiostatin binding domain (871-1005) allows the binding to angiostatin. Belongs to the angiomotin family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 118 kDa
Predicted: 119 kDa
Predicted: 119 kDa
NCBI Official Full Name
angiomotin isoform 1
NCBI Official Synonym Full Names
angiomotin
NCBI Official Symbol
AMOT
NCBI Protein Information
angiomotin
UniProt Protein Name
Angiomotin
Protein Family
UniProt Gene Name
AMOT
UniProt Synonym Gene Names
KIAA1071
NCBI Description
This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions.
Research Articles on AMOT
1. Angiomotin promotes prostate cancer cell proliferation by signaling through the Hippo-YAP pathway.
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Product Notes
The AMOT amot (Catalog #AAA9609525) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AMOT Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AMOT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the AMOT amot for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMOT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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