Immunofluorescence (IF)
(MBS9609221 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit anti-Human, Mouse Collagen IX alpha1 Polyclonal Antibody | anti-COL9A1 antibody
Collagen IX alpha1 Antibody
Gene Names
COL9A1; MED; EDM6; STL4; DJ149L1.1.2
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Collagen IX alpha1; Polyclonal Antibody; Collagen IX alpha1 Antibody; alpha 1 IX collagen chain; Alpha 1 type IX collagen; alpha-1(IX) collagen chain; Cartilage specific short collagen; Collagen alpha 1 IX chain; Collagen alpha-1(IX) chain; Collagen cartilage-specific short alpha collagen; Collagen cartilage-specific short alpha-1(IX) collagen chain; Collagen IX alpha 1 polypeptide; Collagen IX; alpha 1 polypeptide; collagen; type IX alpha; type IX; alpha 1; DJ149L1.1.2; EDM 6; EDM6; FLJ40263; MED; STL 4; STL4; anti-COL9A1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Collagen IX alpha1 antibody detects endogenous levels of total Collagen IX alpha1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
921
Applicable Applications for anti-COL9A1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human Collagen IX alpha1
Subcellular Location
Cytoplasmic
Predicted Cross Reactivity
Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Bovine (88%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9609221 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9609221 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis of Collagen IX alpha1 using LOVO whole cell lysates)
Western Blot (WB)
(Western blot analysis of Collagen IX alpha1 using LOVO whole cell lysates)
Related Product Information for anti-COL9A1 antibody
Function: Structural component of hyaline cartilage and vitreous of the eye.
Subunit Structure: Heterotrimer of an alpha 1(IX), an alpha 2(IX) and an alpha 3(IX) chain.
Post-translational Modifications: Covalently linked to the telopeptides of type II collagen by lysine-derived cross-links. Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Similarity: Each subunit is composed of three triple-helical domains interspersed with non-collagenous domains. The globular domain at the N-terminus of type IX collagen molecules represents the NC4 domain which may participate in electrostatic interactions with polyanionic glycosaminoglycans in cartilage. Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
Subunit Structure: Heterotrimer of an alpha 1(IX), an alpha 2(IX) and an alpha 3(IX) chain.
Post-translational Modifications: Covalently linked to the telopeptides of type II collagen by lysine-derived cross-links. Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Similarity: Each subunit is composed of three triple-helical domains interspersed with non-collagenous domains. The globular domain at the N-terminus of type IX collagen molecules represents the NC4 domain which may participate in electrostatic interactions with polyanionic glycosaminoglycans in cartilage. Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 92 kDa
Predicted: 92 kDa
Predicted: 92 kDa
NCBI Official Full Name
collagen alpha-1(IX) chain isoform 1
NCBI Official Synonym Full Names
collagen type IX alpha 1 chain
NCBI Official Symbol
COL9A1
NCBI Official Synonym Symbols
MED; EDM6; STL4; DJ149L1.1.2
NCBI Protein Information
collagen alpha-1(IX) chain
UniProt Protein Name
Collagen alpha-1(IX) chain
Protein Family
UniProt Gene Name
COL9A1
NCBI Description
This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Structural component of hyaline cartilage and vitreous of the eye.
Research Articles on COL9A1
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Product Notes
The COL9A1 col9a1 (Catalog #AAA9609221) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Collagen IX alpha1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen IX alpha1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000-3000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the COL9A1 col9a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen IX alpha1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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