Western Blot (WB)
(Western blot analysis of Phospho-OSR1 (Thr185) using K562 whole cell lysates)
Rabbit OSR1 Polyclonal Antibody | anti-OSR1 antibody
Phospho-OSR1 (Thr185) Antibody
Gene Names
OXSR1; OSR1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Synonyms
OSR1; Polyclonal Antibody; Phospho-OSR1 (Thr185) Antibody; 2210022N24Rik; 2810422B09Rik; AI462649; AW209236; KIAA1101; mKIAA1101; OSR 1; OTTHUMP00000209062; Oxidative stress responsive 1; Oxidative stress responsive 1 protein; Oxidative stress-responsive 1 protein; OXSR 1; OXSR1; OXSR1_HUMAN; Serine/threonine protein kinase OSR1; Serine/threonine-protein kinase OSR1; anti-OSR1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-OSR1 (Thr185) antibody detects endogenous levels of OSR1 only when phosphorylated at Thr185
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
527
Applicable Applications for anti-OSR1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IF/ICC: 1:100-1:500
IHC: 1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human Phospho-OSR1 (Thr185)
Tissue Specificity
Ubiquitously expressed in all tissue examined.
Conjugation
Unconjugated
Epitope
Phospho Thr185
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Phospho-OSR1 (Thr185) using K562 whole cell lysates)
Western Blot (WB)
(Western blot analysis of Phospho-OSR1 (Thr185) using K562 whole cell lysates)
Immunohistochemistry (IHC)
(MBS9609059 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9609059 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunofluorescence (IF)
(MBS9609059 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9609059 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-OSR1 antibody
Function: Regulates downstream kinases in response to environmental stress. May also have a function in regulating the actin cytoskeleton.
Subunit Structure: Binds to and phosphorylates PAK1. Interacts with chloride channel proteins SLC12A6 isoform 2, SLC12A1 and SLC12A2 but not with SLC12A4 and SLC12A7, possibly establishing sensor/signaling modules that initiate the cellular response to environmental stress. Binds to and phosphorylates RELL1, RELL2 AND RELT.
Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Subunit Structure: Binds to and phosphorylates PAK1. Interacts with chloride channel proteins SLC12A6 isoform 2, SLC12A1 and SLC12A2 but not with SLC12A4 and SLC12A7, possibly establishing sensor/signaling modules that initiate the cellular response to environmental stress. Binds to and phosphorylates RELL1, RELL2 AND RELT.
Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 58 kDa
Predicted: 59 kDa
Predicted: 59 kDa
NCBI Official Full Name
serine/threonine-protein kinase OSR1
NCBI Official Synonym Full Names
oxidative stress responsive kinase 1
NCBI Official Symbol
OXSR1
NCBI Official Synonym Symbols
OSR1
NCBI Protein Information
serine/threonine-protein kinase OSR1
UniProt Protein Name
Serine/threonine-protein kinase OSR1
Protein Family
UniProt Gene Name
OXSR1
NCBI Description
The product of this gene belongs to the Ser/Thr protein kinase family of proteins. It regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton. [provided by RefSeq, Jul 2008]
Uniprot Description
Phosphorylates RELL1, RELL2 and RELT (PubMed:16389068, PubMed:28688764). Phosphorylates PAK1 (PubMed:14707132). Phosphorylates PLSCR1 in the presence of RELT (PubMed:22052202).
Research Articles on OSR1
Similar Products
Product Notes
The OSR1 oxsr1 (Catalog #AAA9609059) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-OSR1 (Thr185) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's OSR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the OSR1 oxsr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OSR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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