Rabbit anti-Human, Mouse RNF19A Polyclonal Antibody | anti-RNF19A antibody

RNF19A Antibody

Cat. Num. AAA9609034

• Gene Names: RNF19A; RNF19
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: RNF19A; Polyclonal Antibody; RNF19A Antibody; AA032313; DKFZP566B1346; Dorfin; Double ring finger protein; Double ring-finger protein; E3 ubiquitin-protein ligase RNF19A; p38; p38 protein; Ring finger protein 19; RING finger protein 19A; Ring IBR ring domain containing protein Dorfin; RN19A_HUMAN; RNF19; Ubce7ip2; UIP117; XYbp; anti-RNF19A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RNF19A antibody detects endogenous levels of total RNF19A
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 838

• Applicable Applications for anti-RNF19A antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide derived from human RNF19A
• Subcellular Location: Membrane. Cytoplasm > Cytoskeleton > Centrosome. Present in the hyaline inclusion bodies specifically found in motor neurons from amyotrophic lateral sclerosis patients. Present in the Lewy bodies specifically found in neurons from Parkinson disease patients.
• Tissue Specificity: Widely expressed, with highest levels in heart. Ubiquitously expressed in the central nervous system.
• Predicted Cross Reactivity: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
• Similarity: Pig (91%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (82%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of RNF19A using Jurkat whole cell lysates)

Immunofluorescene (IF)

(MBS9609034 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

Related Product Information for anti-RNF19A antibody

Function: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection.
Subunit Structure: Interacts with UBE2L3 and UBE2L6. Interacts with transcription factor Sp1. Interacts with VCP, CASR, SNCAIP and with some SOD1 variants which cause amyotrophic lateral sclerosis, but not with wild-type SOD1.
Similarity: Members of the RBR family are atypical E3 ligases. They interact with the E2 conjugating enzyme UBE2L3 and function like HECT-type E3 enzymes: they bind E2s via the first RING domain, but require an obligate trans-thiolation step during the ubiquitin transfer, requiring a conserved cysteine residue in the second RING domain. Belongs to the RBR family. RNF19 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 525459427
• NCBI GeneID: 25897
• NCBI Accession #: NP_001267468.1
• NCBI GenBank Nucleotide #: NM_001280539.1
• UniProt Accession #: Q9NV58; Q52LG1; Q9H5H9; Q9H8M8; Q9UFG0; Q9UFX6; Q9Y4Y1; A3KCU9
• Molecular Weight: Observed: 90 kDa; Predicted: 91 kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF19A
• NCBI Official Synonym Full Names: ring finger protein 19A, RBR E3 ubiquitin protein ligase
• NCBI Official Symbol: RNF19A
• NCBI Official Synonym Symbols: RNF19
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF19A
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF19A
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF19A
• UniProt Synonym Gene Names: RNF19; Dorfin

NCBI Description

This gene encodes a member of the ring between ring fingers (RBR) protein family, and the encoded protein contains two RING-finger motifs and an in between RING fingers motif. This protein is an E3 ubiquitin ligase that is localized to Lewy bodies, and ubiquitylates synphilin-1, which is an interacting protein of alpha synuclein in neurons. The encoded protein may be involved in amyotrophic lateral sclerosis and Parkinson's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates, such as SNCAIP or CASR. Specifically ubiquitinates pathogenic SOD1 variants, which leads to their proteasomal degradation and to neuronal protection.