Western Blot (WB)
(Western blot analysis of PI4KB using COLO205 whole cell lysates)
Rabbit anti-Human PI4KB Polyclonal Antibody | anti-PI4KB antibody
PI4KB Antibody
Gene Names
PI4KB; NPIK; PI4K92; PIK4CB; PI4KBETA; PI4K-BETA; PI4KIIIBETA
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PI4KB; Polyclonal Antibody; PI4KB Antibody; DKFZp686O1820; FLJ30129; NPIK; Phosphatidylinositol 4 kinase beta; Phosphatidylinositol 4 kinase catalytic beta; Phosphatidylinositol 4 kinase catalytic beta polypeptide; Phosphatidylinositol 4 kinase wortmannin sensitive; Phosphatidylinositol 4-kinase beta; PI4K BETA; PI4K-beta; PI4K92; PI4KB_HUMAN; PI4Kbeta; PI4KIIIbeta; PIK4CB; PtdIns 4 kinase; PtdIns 4-kinase beta; Type III phosphatidylinositol 4 kinase beta; anti-PI4KB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
PI4KB antibody detects endogenous levels of total PI4KB
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
801
Applicable Applications for anti-PI4KB antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human PI4KB
Subcellular Location
Endoplasmic Reticulum; Mitochondrion; Golgi Apparatus
Tissue Specificity
Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver.
Predicted Cross Reactivity
Bovine, Horse, Sheep, Dog
Similarity
Bovine (100%), Horse (100%), Sheep (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of PI4KB using COLO205 whole cell lysates)
Western Blot (WB)
(Western blot analysis of PI4KB using COLO205 whole cell lysates)
Related Product Information for anti-PI4KB antibody
Function: Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1, 4, 5, -trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity).
Subunit Structure: Interacts with ARF1 and ARF3 in the Golgi complex, but not with ARF4, ARF5 or ARF6 (PubMed:17555535). Interacts with NCS1/FREQ in a calcium-independent manner. Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner; this interaction competes with NCS1/FREQ binding (By similarity). Interacts with ACBD3 (PubMed:27009356, PubMed:27989622, PubMed:23572552). Interacts with YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ, YWHAZ and SFN (PubMed:23572552).
Similarity: Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.
Subunit Structure: Interacts with ARF1 and ARF3 in the Golgi complex, but not with ARF4, ARF5 or ARF6 (PubMed:17555535). Interacts with NCS1/FREQ in a calcium-independent manner. Interacts with CALN1/CABP8 and CALN2/CABP7; in a calcium-dependent manner; this interaction competes with NCS1/FREQ binding (By similarity). Interacts with ACBD3 (PubMed:27009356, PubMed:27989622, PubMed:23572552). Interacts with YWHAB, YWHAE, YWHAG, YWHAH, YWHAQ, YWHAZ and SFN (PubMed:23572552).
Similarity: Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 91 kDa
Predicted: 92 kDa
Predicted: 92 kDa
NCBI Official Full Name
phosphatidylinositol 4-kinase beta isoform 2
NCBI Official Synonym Full Names
phosphatidylinositol 4-kinase beta
NCBI Official Symbol
PI4KB
NCBI Official Synonym Symbols
NPIK; PI4K92; PIK4CB; PI4KBETA; PI4K-BETA; PI4KIIIBETA
NCBI Protein Information
phosphatidylinositol 4-kinase beta
UniProt Protein Name
Phosphatidylinositol 4-kinase beta
Protein Family
UniProt Gene Name
PI4KB
UniProt Synonym Gene Names
PIK4CB; PI4K-beta; PI4Kbeta; PtdIns 4-kinase beta
Uniprot Description
Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking ().
Research Articles on PI4KB
Similar Products
Product Notes
The PI4KB pi4kb (Catalog #AAA9608600) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PI4KB Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PI4KB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the PI4KB pi4kb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI4KB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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