Rabbit OAS1 Polyclonal Antibody | anti-OAS1 antibody

OAS1 Antibody

Cat. Num. AAA9608337

• Gene Names: OAS1; OIAS; IFI-4; OIASI; E18/E16
• Reactivity: Human, Mouse, Rat; Predicted: Pig
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: OAS1; Polyclonal Antibody; OAS1 Antibody; (2 5')oligo(A) synthetase 1; (2-5'')oligo(A) synthase 1; 2 5 Oligoadenylate Synthetase 1; 2 5' oligo A synthase 1; 2 5' oligo A synthetase 1; 2 5A synthase 1; 2 5A synthetase 1; 2' 5' oligo A synthetase 1; 2' 5' oligoadenylate synthetase 1 40/46kDa; 2' 5' oligoadenylate synthetase 1; 2' 5' oligoisoadenylate synthetase 1; 2''-5''-oligoadenylate synthase 1; 2'5' oligo A synthetase 1; 2'5' oligoadenylate synthetase 1; 2'5' oligoisoadenylate synthetase 1; 2-5A synthase 1; E18/E16; IFI 4; IFI4; OAS 1; OAS1_HUMAN; OIAS; OIASI; p46/p42 OAS; anti-OAS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: OAS1 antibody detects endogenous levels of total OAS1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 414

• Applicable Applications for anti-OAS1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human OAS1
• Subcellular Location: Mitochondrion. Nucleus. Microsome. Endoplasmic Reticulum. Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions.
• Tissue Specificity: By type I interferon (IFN) and viruses.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of OAS1 using HuvEc whole lysates.)

Related Product Information for anti-OAS1 antibody

Function: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L.
Subunit Structure: Monomer (PubMed:9407111). Homotetramer (PubMed:9407111, PubMed:23319625).
Similarity: Belongs to the 2-5A synthase family.

NCBI and Uniprot Product Information

• NCBI GI #: 74229015
• NCBI GeneID: 4938
• NCBI Accession #: NP_001027581.1
• NCBI GenBank Nucleotide #: NM_001032409.2
• UniProt Accession #: P00973; P04820; P29080; P29081; P78485; P78486; Q16700; Q16701; Q1PG42; Q3ZM01; Q53GC5; A8K4N8
• Molecular Weight: Observed: 46 kDa; Predicted: 47 kDa
• NCBI Official Full Name: 2'-5'-oligoadenylate synthase 1 isoform 3
• NCBI Official Synonym Full Names: 2'-5'-oligoadenylate synthetase 1
• NCBI Official Symbol: OAS1
• NCBI Official Synonym Symbols: OIAS; IFI-4; OIASI; E18/E16
• NCBI Protein Information: 2'-5'-oligoadenylate synthase 1
• UniProt Protein Name: 2'-5'-oligoadenylate synthase 1
• Protein Family: 2'-5'-oligoadenylate synthase
• UniProt Gene Name: OAS1
• UniProt Synonym Gene Names: OIAS; (2-5')oligo(A) synthase 1; 2-5A synthase 1

NCBI Description

This gene is induced by interferons and encodes a protein that synthesizes 2',5'-oligoadenylates (2-5As). This protein activates latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. Alternative splicing results in multiple transcript variants with different enzymatic activities. Polymorphisms in this gene have been associated with susceptibility to viral infection and diabetes mellitus, type 1. A disease-associated allele in a splice acceptor site influences the production of the p46 splice isoform. This gene is located in a cluster of related genes on chromosome 12. [provided by RefSeq, Feb 2016]

Uniprot Description

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L.