Western Blot (WB)
(Western blot analysis of IPO9 using COLO205 whole lysates.)
Rabbit anti-Human IPO9 Polyclonal Antibody | anti-IPO9 antibody
IPO9 Antibody
Gene Names
IPO9; Imp9
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
IPO9; Polyclonal Antibody; IPO9 Antibody; Imp9; Imp9a; Imp9b; Importin-9; Importin-9a; Ipo9; IPO9_HUMAN; Ran-binding protein 9; RanBP9; anti-IPO9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
IPO9 antibody detects endogenous levels of total IPO9
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1041
Applicable Applications for anti-IPO9 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human IPO9
Subcellular Location
Cytoplasm. Nucleus.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of IPO9 using COLO205 whole lysates.)
Western Blot (WB)
(Western blot analysis of IPO9 using COLO205 whole lysates.)
Related Product Information for anti-IPO9 antibody
Function: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP-and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of H2B histone (By similarity), RPS7 and RPL18A. Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains. May also import H2A, H3, H4 histones (By similarity), RPL4 and RPL6.
Subunit Structure: Binds with high affinity to RPS7 and RPL18A. The binding is coupled to RanGTP cycles. May bind H2A, H3, H4 histones (By similarity), RPL4 and RPL6 with low affinity. Interacts with PPP2R1A and PPP2R1B.
Similarity: Belongs to the importin beta family.
Subunit Structure: Binds with high affinity to RPS7 and RPL18A. The binding is coupled to RanGTP cycles. May bind H2A, H3, H4 histones (By similarity), RPL4 and RPL6 with low affinity. Interacts with PPP2R1A and PPP2R1B.
Similarity: Belongs to the importin beta family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 115 kDa
Predicted: 116 kDa
Predicted: 116 kDa
NCBI Official Full Name
importin-9
NCBI Official Synonym Full Names
importin 9
NCBI Official Symbol
IPO9
NCBI Official Synonym Symbols
Imp9
NCBI Protein Information
importin-9
UniProt Protein Name
Importin-9
Protein Family
UniProt Gene Name
IPO9
UniProt Synonym Gene Names
IMP9; KIAA1192; RANBP9; Imp9; RanBP9
Uniprot Description
Functions in nuclear protein import as nuclear transport receptor (PubMed:11823430). Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:11823430). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed:11823430). At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran (PubMed:11823430). The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed:11823430). Mediates the nuclear import of RPS7, RPL18A, RPL6, histone H2A, histone H2B and histone (PubMed:11823430). Prevents the cytoplasmic aggregation of RPS7 and RPL18A by shielding exposed basic domains (PubMed:11823430). Mediates the nuclear import of actin ().
Research Articles on IPO9
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Product Notes
The IPO9 ipo9 (Catalog #AAA9608272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IPO9 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IPO9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the IPO9 ipo9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IPO9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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