Rabbit RHO Polyclonal Antibody | anti-RHO antibody

RHO Antibody

Cat. Num. AAA9606498

• Gene Names: RHO; RP4; OPN2; CSNBAD1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: RHO; Polyclonal Antibody; RHO Antibody; CSNBAD1; MGC138309; MGC138311; OPN 2; OPN2; opsd; OPSD_HUMAN; opsin 2; Opsin 2 rod pigment; Opsin-2; Opsin2; Retinitis Pigmentosa 4; Retinitis pigmentosa 4 autosomal dominant; Rhodopsin; RP 4; RP4; anti-RHO antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RHO antibody detects endogenous levels of total RHO
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 348

• Applicable Applications for anti-RHO antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthesized peptide
• Subcellular Location: Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
• Tissue Specificity: Rod shaped photoreceptor cells which mediate vision in dim light.
• Predicted Cross Reactivity: Pig, Rabbit, Dog
• Similarity: Pig (83%), Rabbit (92%), Dog (83%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from A549 cells, using RHO antibody.)

Related Product Information for anti-RHO antibody

Function: Photoreceptor required for image-forming vision at low light intensity (PubMed:8107847, PubMed:7846071). Required for photoreceptor cell viability after birth (PubMed:2215617, PubMed:12566452). Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins (PubMed:8107847, PubMed:28524165, PubMed:26200343, PubMed:28753425). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:28524165, PubMed:26200343).
Subunit Structure: Homodimer (By similarity). Interacts (phosphorylated form) with SAG (PubMed:28524165, PubMed:26200343, PubMed:28753425). Interacts with GNAT1 (PubMed:26200343). Interacts with GNAT3. SAG and G-proteins compete for a common binding site (By similarity). Interacts with GRK1 (PubMed:28524165).
Post-translational Modifications: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region (By similarity). After activation by light, phosphorylated by GRK1 (in vitro) (PubMed:28524165). Contains one covalently linked retinal chromophore. Upon light absorption, the covalently bound 11-cis-retinal is converted to all-trans-retinal. After hydrolysis of the Schiff base and release of the covalently bound all-trans-retinal, active rhodopsin is regenerated by binding of a fresh molecule of 11-cis-retinal(PubMed:12566452).
Similarity: Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 4506527
• NCBI GeneID: 6010
• NCBI Accession #: NP_000530.1
• NCBI GenBank Nucleotide #: NM_000539.3
• UniProt Accession #: P08100; Q16414; Q2M249
• Molecular Weight: Observed: 38 kD; Predicted: 39 kDa
• NCBI Official Full Name: rhodopsin
• NCBI Official Synonym Full Names: rhodopsin
• NCBI Official Symbol: RHO
• NCBI Official Synonym Symbols: RP4; OPN2; CSNBAD1
• NCBI Protein Information: rhodopsin
• UniProt Protein Name: Rhodopsin
• Protein Family: Rhotekin
• UniProt Gene Name: RHO
• UniProt Synonym Gene Names: OPN2

NCBI Description

The protein encoded by this gene is found in rod cells in the back of the eye and is essential for vision in low-light conditions. The encoded protein binds to 11-cis retinal and is activated when light hits the retinal molecule. Defects in this gene are a cause of congenital stationary night blindness. [provided by RefSeq, Aug 2017]

Uniprot Description

Photoreceptor required for image-forming vision at low light intensity (PubMed:8107847, PubMed:7846071). Required for photoreceptor cell viability after birth (PubMed:2215617, PubMed:12566452). Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins (PubMed:8107847, PubMed:28524165, PubMed:26200343, PubMed:28753425). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:28524165, PubMed:26200343).