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Testing Data #2 (Western blot analysis of extracts from A549 cells, using RHO antibody.)

RHO Antibody | anti-RHO antibody

RHO Antibody

Gene Names
RHO; RP4; OPN2; CSNBAD1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
RHO; RHO Antibody; CSNBAD1; MGC138309; MGC138311; OPN2; OPSD; opsin 2; rod pigment; Opsin-2; retinitis pigmentosa 4; autosomal dominant; Rhodopsin; rhodopsin (opsin 2; rod pigment) (retinitis pigmentosa 4; autosomal dominant); RP4; anti-RHO antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
RHO antibody detects endogenous levels of total RHO protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
348
Applicable Applications for anti-RHO antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized peptide derived from Internal of human RHO.
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data #2

(Western blot analysis of extracts from A549 cells, using RHO antibody.)

Testing Data #2 (Western blot analysis of extracts from A549 cells, using RHO antibody.)
References
Nathans J., Proc. Natl. Acad. Sci. U.S.A. 81:4851-4855(1984). Suwa M., Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases. Bechtel S., BMC Genomics 8:399-399(2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
38,893 Da
NCBI Official Full Name
rhodopsin
NCBI Official Synonym Full Names
rhodopsin
NCBI Official Symbol
RHO
NCBI Official Synonym Symbols
RP4; OPN2; CSNBAD1
NCBI Protein Information
rhodopsin
UniProt Protein Name
Rhodopsin
Protein Family
UniProt Gene Name
RHO
UniProt Synonym Gene Names
OPN2

NCBI Description

The protein encoded by this gene is found in rod cells in the back of the eye and is essential for vision in low-light conditions. The encoded protein binds to 11-cis retinal and is activated when light hits the retinal molecule. Defects in this gene are a cause of congenital stationary night blindness. [provided by RefSeq, Aug 2017]

Uniprot Description

Photoreceptor required for image-forming vision at low light intensity (PubMed:8107847, PubMed:7846071). Required for photoreceptor cell viability after birth (PubMed:2215617, PubMed:12566452). Light-induced isomerization of the chromophore 11-cis-retinal to all-trans-retinal triggers a conformational change that activates signaling via G-proteins (PubMed:8107847, PubMed:28524165, PubMed:26200343, PubMed:28753425). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:28524165, PubMed:26200343).

Research Articles on RHO

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Product Notes

The RHO rho (Catalog #AAA855408) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The RHO Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RHO can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the RHO rho for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RHO, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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