Immunofluorescence (IF)
(MBS9605995 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human, Mouse SFRS11 Polyclonal Antibody | anti-SFRS11 antibody
SFRS11 Antibody
Gene Names
SRSF11; p54; NET2; SFRS11; dJ677H15.2
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SFRS11; Polyclonal Antibody; SFRS11 Antibody; Arginine rich 54 kDa nuclear protein; Arginine-rich 54 kDa nuclear protein; arginine/serine-rich 11; dJ677H15.2; DKFZp686M13204; NET2; P54; Serine/arginine-rich splicing factor 11; SFR11; SFRS 11; Splicing factor; Splicing factor arginine/serine rich 11; Splicing factor p54; SRS11_HUMAN; SRSF11; anti-SFRS11 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
SFRS11 antibody detects endogenous levels of total SFRS11
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
483
Applicable Applications for anti-SFRS11 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Nucleus. Colocalizes with spliceosome components.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (92%), Chicken (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9605995 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9605995 staining LOVO by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from LOVO cells using SFRS11 antibody.The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from LOVO cells using SFRS11 antibody.The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-SFRS11 antibody
Function: May function in pre-mRNA splicing.
Subunit Structure: Interacts with PUF60.
Similarity: Belongs to the splicing factor SR family.
Subunit Structure: Interacts with PUF60.
Similarity: Belongs to the splicing factor SR family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 54 kD
Predicted: 54 kDa
Predicted: 54 kDa
NCBI Official Full Name
serine/arginine-rich splicing factor 11 isoform 2
NCBI Official Synonym Full Names
serine and arginine rich splicing factor 11
NCBI Official Symbol
SRSF11
NCBI Official Synonym Symbols
p54; NET2; SFRS11; dJ677H15.2
NCBI Protein Information
serine/arginine-rich splicing factor 11
UniProt Protein Name
Serine/arginine-rich splicing factor 11
UniProt Gene Name
SRSF11
UniProt Synonym Gene Names
SFRS11; p54
NCBI Description
This gene encodes 54-kD nuclear protein that contains an arginine/serine-rich region similar to segments found in pre-mRNA splicing factors. Although the function of this protein is not yet known, structure and immunolocalization data suggest that it may play a role in pre-mRNA processing. Alternative splicing results in multiple transcript variants encoding different proteins. In addition, a pseudogene of this gene has been found on chromosome 12.[provided by RefSeq, Sep 2010]
Uniprot Description
May function in pre-mRNA splicing.
Research Articles on SFRS11
Similar Products
Product Notes
The SFRS11 srsf11 (Catalog #AAA9605995) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SFRS11 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SFRS11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the SFRS11 srsf11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SFRS11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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