Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells using MAP4K3 antibody.)
Rabbit MAP4K3 Polyclonal Antibody | anti-MAP4K3 antibody
MAP4K3 Antibody
Gene Names
MAP4K3; GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MAP4K3; Polyclonal Antibody; MAP4K3 Antibody; Germinal center kinase like kinase; Germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MAPKKKK3; MEK kinase kinase 3; MEKKK 3; MEKKK3; mitogen-activated protein kinase kinase kinase kinase 3; RAB8IPL1; anti-MAP4K3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MAP4K3 antibody detects endogenous levels of total MAP4K3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
873
Applicable Applications for anti-MAP4K3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasmic and Nuclear
Tissue Specificity
Ubiquitously expressed in all tissues examined, with high levels in heart, brain, placenta, skeletal muscle, kidney and pancreas and lower levels in lung and liver.
Predicted Cross Reactivity
Pig, Bovine, Dog
Similarity
Pig (100%), Bovine (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells using MAP4K3 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells using MAP4K3 antibody.)
Immunofluorescene (IF)
(MBS9604803 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9604803 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-MAP4K3 antibody
Function: May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.
Subunit Structure: Interacts with SH3GL2. Interaction appears to regulate MAP4K3-mediated JNK activation (By similarity).
Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Subunit Structure: Interacts with SH3GL2. Interaction appears to regulate MAP4K3-mediated JNK activation (By similarity).
Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 101 kD
Predicted: 102 kDa
Predicted: 102 kDa
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase kinase 3 isoform 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase kinase 3
NCBI Official Symbol
MAP4K3
NCBI Official Synonym Symbols
GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase kinase 3
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase kinase 3
Protein Family
UniProt Gene Name
MAP4K3
UniProt Synonym Gene Names
RAB8IPL1; GLK; MEK kinase kinase 3; MEKKK 3
NCBI Description
This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
Uniprot Description
May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway.
Research Articles on MAP4K3
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Product Notes
The MAP4K3 map4k3 (Catalog #AAA9604803) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAP4K3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAP4K3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the MAP4K3 map4k3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP4K3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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