Immunofluorescence (IF)
(MBS9604552 staining A549 cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blockedin 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab (MBS9604552) and mouse anti-betatubulin Ab MBS576566 for 1 hour at 37°C. An AlexaFluor 594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Rabbit MYF5 Polyclonal Antibody | anti-MYF5 antibody
MYF5 Antibody
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
IF/ICC: 1:100 - 1:500
IHC: 1:50 - 1:200
EIA: 1:20000 - 1:40000
Immunofluorescence (IF)
(MBS9604552 staining A549 cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blockedin 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab (MBS9604552) and mouse anti-betatubulin Ab MBS576566 for 1 hour at 37°C. An AlexaFluor 594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9604552 staining A549 cells by IF/ICC. The samples were fixedwith PFA and permeabilized in 0.1% Triton X-100,then blockedin 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab (MBS9604552) and mouse anti-betatubulin Ab MBS576566 for 1 hour at 37°C. An AlexaFluor 594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9604552 at 1/100 staining Rat testis tissue by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4° Covernight. An HRP conjugated anti-Rabbit antibody was usedas the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9604552 at 1/100 staining Rat testis tissue by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample wasthen blocked and incubated with the primary antibody at 4° Covernight. An HRP conjugated anti-Rabbit antibody was usedas the secondary antibody.)
Western BLot (WB)
(Western blot analysis of extracts from various samples, usingMYF5 Antibody.Lane 1: Mouse brain lysatesLane 2: Mouse muscle lysatesLane 3: Mouse liver lysatesLane 4: Rat brain lysates)
Western BLot (WB)
(Western blot analysis of extracts from various samples, usingMYF5 Antibody.Lane 1: Mouse brain lysatesLane 2: Mouse muscle lysatesLane 3: Mouse liver lysatesLane 4: Rat brain lysates)
Western Blot
(Western blot analysis of extracts from Rat kidney, using MYF5 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot
(Western blot analysis of extracts from Rat kidney, using MYF5 Antibody. The lane on the left was treated with blocking peptide.)
NCBI and Uniprot Product Information
Uniprot Description
Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYOG and MYOD1, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.
Research Articles on MYF5
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Product Notes
The MYF5 myf5 (Catalog #AAA9604552) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYF5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MYF5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA (Peptide). WB: 1:500 - 1:1000 IF/ICC: 1:100 - 1:500 IHC: 1:50 - 1:200 EIA: 1:20000 - 1:40000. Researchers should empirically determine the suitability of the MYF5 myf5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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