Western Blot (WB)
(Western blot analysis of GBP2 expression in human liver tissue lysate ;, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse GBP2 Polyclonal Antibody | anti-GBP2 antibody
GBP2 Antibody
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
GBP2; Polyclonal Antibody; GBP2 Antibody; GBP 2; GTP binding protein 2; Guanine nucleotide binding protein 2; Guanylate binding protein 2 interferon inducible; HuGBP 2; HuGBP2; Interferon induced guanylate binding protein 2; anti-GBP2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
GBP2 antibody detects endogenous levels of total GBP2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
591
Applicable Applications for anti-GBP2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane; Lipid-Anchor; Cytoplasmic Side
Tissue Specificity
By IFNG/IFN-gamma during macrophage activation, and by TNF and IL1B.
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of GBP2 expression in human liver tissue lysate ;, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of GBP2 expression in human liver tissue lysate ;, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9604013 at 1/100 staining Human cervical cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604013 at 1/100 staining Human cervical cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-GBP2 antibody
Function: Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions, but the major reaction product is GDP (PubMed:8706832). Exhibits antiviral activity against influenza virus. Promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes (By similarity).
Subunit Structure: Homodimer; homodimerization occurs upon GTP-binding and is required for the association with membranous structures. Heterodimer with other family members, including GBP1, GBP3, GBP4 and GBP5.
Post-translational Modifications: Isoprenylation is required for proper subcellular location.
Similarity: Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3-type GTPase family. GB1 subfamily.
Subunit Structure: Homodimer; homodimerization occurs upon GTP-binding and is required for the association with membranous structures. Heterodimer with other family members, including GBP1, GBP3, GBP4 and GBP5.
Post-translational Modifications: Isoprenylation is required for proper subcellular location.
Similarity: Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3-type GTPase family. GB1 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 67 kDa
Predicted: 68 kDa
Predicted: 68 kDa
NCBI Official Full Name
guanylate-binding protein 2
NCBI Official Synonym Full Names
guanylate binding protein 2
NCBI Official Symbol
GBP2
NCBI Protein Information
guanylate-binding protein 2
UniProt Protein Name
Guanylate-binding protein 2
Protein Family
UniProt Gene Name
GBP2
UniProt Synonym Gene Names
GBP-2; HuGBP-2
NCBI Description
This gene belongs to the guanine-binding protein (GBP) family, which includes interferon-induced proteins that can bind to guanine nucleotides (GMP, GDP and GTP). The encoded protein is a GTPase which hydrolyzes GTP, predominantly to GDP. The protein may play a role as a marker of squamous cell carcinomas. [provided by RefSeq, Jul 2013]
Uniprot Description
Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions, but the major reaction product is GDP (PubMed:8706832). Exhibits antiviral activity against influenza virus. Promote oxidative killing and deliver antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes ().
Research Articles on GBP2
Similar Products
Product Notes
The GBP2 gbp2 (Catalog #AAA9604013) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GBP2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GBP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the GBP2 gbp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GBP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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